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原因不明复发性流产母胎界面调节性T细胞对巨噬细胞的调控 被引量:1

The regulation of decidual regulatory T cells on macrophages in unexplained recurrent spontaneous abortion patients
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摘要 目的探讨原因不明复发性流产(URSA)患者蜕膜组织中CD4+CD25+调节性T(Treg)细胞对巨噬细胞(Mφ)的调控作用。方法选择2008年3月至2009年4月在上海交通大学医学院附属仁济医院就诊的URSA患者(URSA组)及正常早孕人工流产妇女(正常组)各15例,采集蜕膜组织,免疫磁珠法分选Treg细胞和Mφ。将两组Mφ单独培养,同时URSA组Mφ分别与正常组、URSA组Treg细胞共同培养(直接、培养液中加入抗TGF-β抗体和Transwell体系)6d,检测MφCD80、CD86、IL-10和IFN-γ的表达。结果 (1)与正常组相比,URSA组MφCD80、CD86表达显著增高;MφIL-10表达显著降低(P<0.05);IFN-γ的表达差异无统计学意义(P>0.05)。(2)两组Treg细胞均能下调MφCD80、CD86和IFN-γ表达,上调IL-10的表达;正常组Treg调控Mφ的能力显著高于URSA组(P<0.05)。在含有anti-TGF-β的培养液或在Transwell体系中共培养后,Treg细胞丧失调控Mφ的能力。结论 URSA患者蜕膜中Treg细胞对Mφ调控失调,从而导致固有免疫异常所致的获得性免疫失调。 Objective To investigate the regulation of CD4 +CD25 + T cells (Tregs) on macropaghs in deciduas in unexplained recurrent spontaneous abortion (URSA) patients. Methods Monocytes and Tregs were isolated from decidua of URSA ( n = 15 ) and normal, early pregnant women ( n = 15 ) by magnetic cell separation and co-cultured for 6 days. The regulation of Tregs on macrophages was assessed in vitro co-cultures (with or without anti-TGF-β and transwell experiments). Expression of MφCD80, CD86, IL-10 and IFN-γ was measured by FCM or ELISA. Results The expression of MφCD80, CD86 were higher whereas the expression of MφIL-10 was lower in decidua in URSA patients compared to controis; while the expression of MφIFN-γ did not differ between the two groups. In vitro ,Tregs could inhibit the expression of MφCD80, CD86 and IFN-γ; increase the expression of MφIL-10. Tregs, as incubated in the medium contained TGF-β or in the transwell system,would lost its regulatory ability. Conclusion The regulatory activity of Tregs to Mφs are decreased in unexplained RSA patients.
出处 《中国实用妇科与产科杂志》 CAS CSCD 北大核心 2010年第8期597-599,共3页 Chinese Journal of Practical Gynecology and Obstetrics
基金 国家自然科学基金重点项目(30530740) 上海市教委第五期重点学科(G50204)
关键词 流产 调节性T细胞 巨噬细胞 共刺激分子 白介素-10 干扰素-γ abortion regulatory T cells macrophage costimulatory molecules interlukin-10 interferon-γ
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