绒癌细胞系中Fas/FasL的表达及免疫逃逸机制探讨

The Expression of Fas/FasL and its mechanism of immune escape in the choriocarcinoma cell lines

目的观察Fas/FasL系统在绒毛膜癌(绒癌)细胞系(JEG-3、BeWo)和T细胞系(Jurkat)中的表达,探讨Jurkat细胞凋亡的机制及Fas/FasL系统与人绒癌细胞免疫逃逸及反杀伤作用的关系。方法 RT-PCR检测绒癌细胞系JEG-3、BeWo与Jurkat细胞中Fas/FasL mRNA表达,免疫印迹实验检测3个细胞系中Fas/FasL的蛋白表达情况,台盼蓝拒染实验检测Jurkat细胞成活率,流式细胞仪检测细胞凋亡。结果与Jurkat细胞相比,在mRNA水平和蛋白水平,绒癌细胞中Fas表达低下,而FasL表达升高。绒癌细胞与Jurkat细胞共培养可诱导Jurkat细胞存活率下降,诱导凋亡(P<0.05),随共培养时间延长Jurkat细胞凋亡率升高(P<0.05)。结论绒癌细胞能抵抗Fas/FasL系统介导的凋亡,高表达的FasL可反击杀伤Fas+T细胞,低表达的Fas可以逃避T细胞的免疫杀伤。

Objective To observe the expression of Fas / FasL in choriocarcinoma cell lines （JEG-3 and BeWo） ,its apoptosis and immune escape mechanism. Methods RT-PCR method was used to detect the mRNA expression of Fas / FasL in JEG-3, BeWo and Jurkat cell lines, Western blot assay was used to detect Fas / FasL protein expression. Trypan blue dye assay was used to detect the survival rate of Jurkat cells. Flow cytometry （FCM） was used to monitor the cell apoptosis. Results Compared with Jurkat cells, the expression of Fas mRNA was lower ,while FasL mRNA was higher in choriocarcinoma cell lines with a significant difference. Co-cultured with Jurkat ceils , choriocarcinoma cells could induce Jurkat cell apoptosis （ P 〈 0. 05 ）, with extended culture time, the apoptosis rate of Jurkat cells was notably increased（P 〈 0. 05 ）. Conclusion The choriocarcinoma cell can resist the apoptosis induced by Fas / FasL system, the higher expression of FasL can inhibit immune function, and the lower Fas expression in T cells can evade immune destrnction.