鳕鱼皮胶原蛋白肽酶解液脱色脱腥工艺的研究

Study on decolorizing and deodorization of cod skin enzymolysis collagen peptide liquid

鱼皮胶原蛋白肽是指鱼皮胶原或明胶经蛋白酶等降解处理后制得的产物。该产物在氨基酸组成方面与大分子的胶原蛋白不相上下,而且更容易被人体吸收,还表现出超出大分子胶原蛋白的生理活性,可广泛应用于食品、医药和保健品等行业。近年来,由于口蹄疫、疯牛病等动物疾病的爆发及宗教等原因,从水产品中提取胶原蛋白或胶原蛋白多肽已成为国内外研究的热点。但胶原蛋白水解产物具有令人不愉快的颜色和气味,限制了其推广应用。本研究以蛋白质损失率和感官值为考察指标,通过单因素和正交实验,研究了活性炭对水解产物脱色脱腥的效果。确定最佳脱色脱腥工艺条件为活性炭用量0.3%(w/v)、温度50℃、时间45min、pH5.5,在此条件下水解产物蛋白质损失率为4.2%,感观值为8,基本达到了脱色脱腥的目的,提高了水解蛋白的品质。

Fish skin collagen peptide refers to the degradation of the collagen or gelatin of the fish skin by the protease.The amino acid composition of the products were comparable with the collagen molecules,but were more easily absorbed by the body.It was also demonstrated the physiological activity of super collagen molecules.They can be widely used in food,medicine and health care products.In recent years,due to the outbreak of animal disease such as foot and mouth disease,mad cow diseases,as well as religious and other reasons,collagen or collagen peptide extracted from aquatic product has become a hot research at home and abroad.However,they often have the unpleasant color and odor which limited its popularization and application.In this paper,the loss rate of protein and sensory value was used as the indexes,through single-factor test and orthogonal test,the effect of decolorizing and deodorization of the enzymolysis products by activated carbon was investigated.Result from this study indicated that the best condition of decolorizing and deodorization was activated carbon 0.3%（w/v）,temperature 50℃,time 45min,pH5.5.The loss ratio of hydrolyzed protein was 4.2% and the sensory value was 8 under the optimal conditions.The quality of hydrolyzed protein was improved.