癫痫大鼠颞叶和海马区PX1mRNA与CX36mRNA表达及甘珀酸的干预

Expression of Px1mRNA and Cx36mRNA in Temporal Lobe Cortex and Hippocampus and Interventional Effect of Carbenoxolone of Pentylenetetrazole-Induced Seizure of Rats

目的观察戊四氮点燃大鼠颞叶和海马区PX1mRNA和CX36mRNA的表达及阻断剂甘珀酸的干预作用。方法 K组、CBX、NS组SD大鼠各10只,K组、CBX组用PTZ点燃,再分别用生理盐水CBX干预3d,NS组注射生理盐水。观察3组大鼠的行为变化,采用RT-PCR方法观察颞叶和海马区PX1mRNA和CX36mRNA的表达。结果①K组,CBX组28d左右均达到点燃,NS组大鼠行为学无变化。②PX1mRNA在海马和颞叶的表达均为K组>CBX组>NS组(P<0.05),各组海马区的表达高于颞叶(P<0.05)。CX36mRNA在海马和颞叶的表达均为K组>CBX组>NS组(P<0.05),各组颞叶的表达高于海马区(P<0.05)。结论 PX1和CX36可能参与癫痫的发生发展,其阻断剂CBX有潜在的抗惊厥作用。

Objective To investigate the expression of pannexin 1mRNA and connexion-36mRNA in temporal lobe cortex and hippocampus of pentylenetetrazole-kindled epileptic rats and the intervention of carbenoxolone.Methods KS、CBX and NS were 10 rats each and CBX group were firstly kindked by PTZ respectively injected NS and CBX for three days the NS group were received NS only.The behavior changes of all rats were observed.The expression of PX1mRNA and CX36mRNA in temporal lobe cortex and hippocampus were detected by RT-PCR.Results ① The K and the CBX groups both achieved ignition after 28 days or so,however,the behavior of the NS group didn＇t change.② The expression of PX1mRNA in temporal lobe cortex and hippocampus was K groupCBX groupNS group （P0.05）,and the expression of each group was higher in the hippocampus than in the temporal lobe cortex （P0.05）.The expression of CX36 mRNA in temporal lobe cortex and hippocampus was K groupCBX groupNS group （P0.05）,and the expression of each group was higher in temporal lobe cortex than hippocampus （P0.05）.Conclusion PX1 and CX36 were closely involved in epileptogenesis,and its gap junction blocker CBX may have anti-epileptic effect potentially.