摘要
目的 对2006年和2008年采自安徽蒙城的间日疟原虫群体遗传结构及其与发病率之间的关系进行研究.方法 以间日疟原虫裂殖子表面基因蛋白3α（Plasmodium vivax merozoite surface protein genes-3α,PvMSP-3α）为分子标志,利用PCR-RFLP技术对不同年度间日疟原虫群体遗传多态现象进行分析,用卡方检验比较年度间等位基因频率是否存在差异.结果 共发现了3种类型的MSP-3α基因.2006年的样本仅发现两个型：多数为A型（91.30%）,少数为C型（4.35%）,并有2例A和C的混合感染（4.35%）;2008年样本中,仅发现A型（88.60%）和B型（11.40%）,无混合感染.PCRRFLP分析MSP-3α基因存在高度多态性.卡方检验显示2006年和2008年间的差异无统计学意义.结论 安徽蒙城的间日疟原虫存在较高的遗传多态性,且遗传多态性未随疟疾疫情的下降而改变.
Objective To study the relationship between the population genetic structure of the Plasmodium vivax and the malaria situation in Anhui Province between different years. Methods P. vivax merozoite surface protein genes-3α （PvMSP-3α） was amplified by PCR-RFLP to analyze the population genetic structure. The allele frequency was compared by chi square test. Results Three different sizes of the MSP3α gene were found, type A（1.9 kb）, type B（1.5 kb） and type C（1.1 kb）. There were type A（91.30%）,C（4.35%）and mixture infection of A and C（4.35%）in 2006, while type A （88.60%）and B（11.40%） in 2008 and most of the samples were type A in the two years. The MSP-3α gene exhibited high polymorphism by PCR-RFLP analysis. The chi square test did not detect significant difference in allele frequencies between the two years. Conclusion The population genetic structure of P. vivax in Anhui Province exhibited high polymorphism, and the polymorphism has not been changed with the decrease of malaria situation.
出处
《国际医学寄生虫病杂志》
CAS
2010年第4期198-201,共4页
International JOurnal of Medical Parasitic Diseases
基金
中国疾病预防控制中心寄生虫病预防控制所所长基金
