弓形虫感染对早孕期人绒毛膜滋养层细胞人类白细胞抗原G表达的影响

Effect of infection with Toxoplasma gondii on the HLA-G expression in the early pregnant human trophoblastic cells

目的 研究弓形虫感染早孕期绒毛膜滋养层细胞中人类白细胞抗原G（human leukocyte antigen G,HLA-C）表达水平的变化,探讨弓形虫感染致不良妊娠的分子免疫机制.方法 选取正常妊娠6～12周妇女行人工流产术后的绒毛组织,分离滋养层细胞.将绒毛膜滋养层细胞分为实验组和对照组,实验组加入1×104个弓形虫,对照组加入等量生理盐水,培养48 h后,采用实时荧光定量PCR检测各组人绒毛膜滋养层细胞HLA-G mRNA表达水平,流式细胞技术检测各组人绒毛膜滋养层细胞中HLA-G阳性细胞亚群数量.结果 实验组和对照组HLA-G mRNA平均表达水平分别为0.15±0.04和0.29±0.07,两组之间的差异具有统计学意义（P＜0.01）;实验组绒毛膜滋养层细胞HLA-G阳性细胞亚群的数量为（12.19±2.04）%,对照组为（24.24±3.07）%,两组之间的差异具有统计学意义（P＜0.01）.结论 早孕期弓形虫感染可下调绒毛膜滋养层细胞HLA-G的表达,减少绒毛膜滋养层细胞中HLA-G阳性细胞亚群数量.

Objective To investigate the effect of infection with Toxoplasma gondii on the human leukocyte antigen G（HLA-G） expression in the human trophoblastic cells and to explore the immune mechanisms in abnormal pregnancy induced by T. gondii. Methods The trophoblastic cells were isolated from the villous tissues, which were obtained from induced abortions of normal pregnancies for 6 to 12 weeks. The trophoblastic cells were divided into experimental and control groups. The experimental group was infected with T. gondii（1 × 104）, and the control group was without infection. After being incubated for 48 h, the expression of HLA-G mRNA was analyzed with quantitative real-time PCR. Results Experimental results showed that the average expression levels of HLA-G mRNA in trophoblastic cells were 0. 15 ＋0.04 in the experimental group,while those in the control group were 0. 29 ± 0. 07. On protein level, the average ratio of HLA-G positive subgroup in the experimental and control group were （12.19 ± 2.04） % and （24.24 ± 3.07） %, respectively.The differences in these groups were statistically significant （P 〈 0.01）. Conclusion From the above observations, it is evident that during the early stage of pregnancy, the expression of HLA-G in the trophoblastic cells was decreased in both mRNA and protein levels due to the infection of T. gondii.