转基因技术在疟原虫药物抗性机制研究中的应用

Investigation of drug-resistant mechanism of malaria parasites by transfection technology

疟疾是世界上危害人类健康最严重的感染性疾病之一.随着抗疟药物的广泛使用,疟原虫抗药性问题日趋严重.开展疟原虫药物抗性机制的研究,有助于开发新的药物以及制定用药策略.采用疟原虫转基因的方法能够从分子水平证实抗性基因与药物敏感的相关性以及基因突变在药物抗性产生中所起的作用.该文综述了疟原虫转基因技术在氯喹和磺胺多辛-乙胺嘧啶药物抗性机制研究中的应用.

Malaria is one of the leading infectious diseases in the world. The acquisition and spread of drug-resistant parasite arose gradually since widely utilization of antimalarial drugs. Investigations of the drugresistant mechanisms of malaria parasites may have advantages in developing new drug and modifying chemotherapy strategy. Introduction of transfection technologies made it possible to prove the role of candidate genes responsible for drug resistance, and the contribution of specific mutations to the resistance phenotype. This review focused on the application of transfection technologies in both Plasmodium falciparum and P. vivax to dissect the mechanisms of antimalarial drug resistance including chloroquine and sulfadoxin-pyrimethamine.