拮抗放线菌Y-71的鉴定及其代谢活性物质的性质

Identification of an Actinomyces Y-71 and Its Metabolic Substances' Property

本研究从云南采集的腐质土壤样品中分离筛选到一株拮抗放线菌Y-71,通过形态特征、培养特征、生理生化特征、细胞化学组分和基于16SrRNA基因序列的相似性分析等研究,鉴定菌株Y-71为脱叶链霉菌(Streptomyces exfoliatus)。抗菌谱测定及稳定性研究结果表明,菌株Y-71发酵液抑菌谱较广,对革兰氏阳性细菌和革兰氏阴性细菌都有较好的抑制作用;其抑菌活性物质对温度敏感,在70°C处理后抑菌活性丧失;在pH2-8条件下稳定;不能被蛋白酶降解,可初步判定此活性物质不属于蛋白质或肽类物质。本研究为该菌株今后的应用、抗生素的分离提纯奠定了基础。

An antimicrobial actinomycete,labelled Y-71,was selected from the putrilage soil collected from Yunnan,China.We identified it Streptomyces exfoliatus,by light microscope and SEM observation,physiological and biochemical characterization,cellwall components analysis and 16S rRNA sequence alignment.Its fermentation liquor had wide antimicrobial spectrum,effectively against the Gram-positive and Gram-negative bacteria.The antimicrobial substance was sensitive to high temperature and the 70°C condition could deprive its antimicrobial activity thoroughly.However,it was rather stable under the 2？8 pH value,and couldn＇t be degraded by proteases.Based on these results,we proposed that its antimicrobial substances may not be proteins or peptides.Our research revealed the basic features of strain Y-71 and may help a lot to its further study and application,such as the isolation and purification of antibiotics.