氟对MC3T3-E1细胞矿化能力的影响

Effect of Fluoride on Mineralization in Mice Osteoblast Cell Line MC3T3-E1 Cells

目的探讨氟对小鼠成骨细胞系MC3T3-E1细胞矿化的影响。方法在4×104个/孔的MC3T3-E1细胞培养液中加入0～10-4mol/L的氟化钠,于培养第14、28天,采用茜素红染色法检测矿化结节的形成情况。结果染毒14天,各氟化钠染毒MC3T3-E1细胞OD值间比较,差异均无统计学意义。与对照组相比,染氟28天时,10-7、10-6mol/L氟化钠染毒组MC3T3-E1细胞OD值较高,10-4mol/L氟化钠染毒组MC3T3-E1细胞OD值较低,差异均有统计学意义(P<0.05)。且随着氟化钠染毒剂量的升高,MC3T3-E1细胞OD值呈先升高后降低的趋势。结论低浓度氟化钠(10-7～10-6mol/L)可促进MC3T3-E1细胞的矿化,而高浓度氟化钠则可抑制MC3T3-E1矿化结节的形成。氟对成骨细胞矿化过程起双向调节作用。

Objective The effect of fluoride on mineralization was examined using a murine osteoblastic cell line, MC3T3-E 1. Methods 4×10^-4 cells/well MC3T3-E1 cells were cultured in medium containing 0-10^-4 mol/L NaF for 14 days or 28 days. Mineralization was examined by alizarin red staining. Results There was no difference in OD values between fluoride-treated cells and control at the 14th day. Alizarin red staining indicated a biphasic pattern of mineralization had been induced in MC3T3- E1 at the 28th day. Mineralization was maximal at 10-64 mol/L NaF and minimal at 10^-4 mol/L NaF （P〈0.05）. Conclusion Low concentration of fluoride（10^-7-10^4 mol/L） may enhance mineralization of MC3T3-E 1 cells, while high concentration of fluoride may inhibit mineralization of MC3T3-E1 cells. Fluoride may play an important role in osteoblast mineralization.