焦化工人尿中多种多环芳烃单羟基代谢物的调查

Levels of Urinary Mono-hydroxylated Metabolites of PAHs in Coke-oven Workers

目的了解焦化工人多种多环芳烃（PAHs）的内暴露水平。方法于2006年11月采集我国南方某焦化厂工人及对照人群尿样。尿液经过酶解、固相萃取（SPE）富集和净化后，采用高效液相色谱．荧光检测方法检测多环芳烃羟基代谢物（HO—PAHs）。结果在尿样中共检测出8种多环芳烃羟基代谢物。除两组人群4．羟基菲浓度中位数接近外，工人组其余HO—PAHs水平均为对照组1倍以上；其中工人组尿中1-羟基芘（1-OHP）浓度是对照组的7倍。吸烟会对受试人群萘和芴的内暴露水平产生显著影响。工人组尿中1-OHP浓度与2-羟基萘（2-HON）、2-羟基芴（2-HOHu）、9-羟基菲（9．HOPhe）、EHOPhe和EHO．PAHs均存在显著的相关性，吸烟和非吸烟工人r值范围分别在0．638—0．998和0．805～0．990之间，但在对照组中，1-OHP与其他尿HO—PAHs的相关性低于工人组，r值在0．436—0．993之间，且1-OHP与2-HON的浓度相关性无统计学意义（r值为0．346）。结论在职业高暴露环境下，1-OHP能很好地指示PAHs内暴露水平；仅用1-OHP并不一定能反映一般人群实际PAHs的暴露水平。

Objective To assess the internal exposure level of several polycyclic aromatic hydrocarbons （PAHs） in coke-oven workers in south China. Methods The urine samples were collected in November, 2006. After enzymic hydrolysis, the urine samples were pre-concentrated and purified in solid phase extraction（SPE ）, and then the urinary hydroxy-PAHs were determined by using high performance liquid chromatography with fluorescence detection. Results Eight hydroxy polycyclic aromatic hydrocarbons were detected in the urine samples. The median concentrations of hydroxy-PAHs （especially for 1-hydroxypyrene） in urine of coke-oven workers were more than one time as more as those of control （and seven times for 1-hydroxypyrene）,except for 4-hydroxyphenanthrene which showed similar levels between tow groups. In addition, the results suggested that smoking had a notable impact on the level of naphthalene and fluorene in urine. There was a significant correlation between 1-OHP and 2-HON, 2-HOFlu, 9-HOPhe, ∑HOPhe and EHO-PAHs among coke-oven workers [the values of correlation coefficient （r） were 0.638- 0.998 for smokers and 0.805-0.990 for non-smokers]. Conclusion 1-OHP may he a good biomarker for the assessment of PAHs exposure in occupational population working in the environment with high exposure level of PAHs, but not for the common people.