Potential coexistence of both bacterial and eukaryotic small RNA biogenesis and functional related protein homologs in Archaea 被引量：1

Potential coexistence of both bacterial and eukaryotic small RNA biogenesis and functional related protein homologs in Archaea

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摘要 RNA silencing plays crucial roles in both bacteria and eukaryotes, yet its machinery appears to differ in these two kingdoms. A couple of Argonaute protein homologs have been reported in some archaeal species in recent years. As Argonaute protein is the key component of eukaryotic RNA silencing pathways, such findings suggested the possibility of existence of eukaryotic RNA silencing like pathways in Archaea, which present the life forms between prokaryotes and eukaryotes. To further explore such hypothesis, we systematically screened 71 fully sequenced archaeal genomes, and identified some proteins containing homologous regions to the functional domains of eukaryotic RNA silencing pathway key proteins. The phylogenetic relationships of these proteins were analyzed. The conserved functional amino acids between archaeal and eukaryotic Piwi domains suggested their fimctional similarity. Our results provide new clues to the evolution of RNA silencing pathways. RNA silencing plays crucial roles in both bacteria and eukaryotes, yet its machinery appears to differ in these two kingdoms. A couple of Argonaute protein homologs have been reported in some archaeal species in recent years. As Argonaute protein is the key component of eukaryotic RNA silencing pathways, such findings suggested the possibility of existence of eukaryotic RNA silencing like pathways in Archaea, which present the life forms between prokaryotes and eukaryotes. To further explore such hypothesis, we systematically screened 71 fully sequenced archaeal genomes, and identified some proteins containing homologous regions to the functional domains of eukaryotic RNA silencing pathway key proteins. The phylogenetic relationships of these proteins were analyzed. The conserved functional amino acids between archaeal and eukaryotic Piwi domains suggested their fimctional similarity. Our results provide new clues to the evolution of RNA silencing pathways.

作者 Yan Li Xiuying Liu Li Huang Huishan Guo Xiu-Jie Wang

机构地区 State Key Laboratory of Plant Genomics and National Center for Plant Gene Research State Key Laboratory of Plant Genomics State Key Laboratory of Microbial Resources Graduate University of Chinese Academy of Sciences

出处《Journal of Genetics and Genomics》 SCIE CAS CSCD 2010年第8期493-503,共11页 遗传学报（英文版）

基金 supported by grants from the Ministry of Science and Technology of China (No. 2006CB101605) the Chinese Academy of Sciences (No. KSCX2-YW- R-134) to X.-J. W. from the National Science Founda-tion of China (No. 90919010) to H. Guo

关键词 ARCHAEA small RNA RNA silencing PIWI RNase Ill Archaea small RNA RNA silencing Piwi RNase Ill

分类号 Q52 [生物学—生物化学] Q756 [生物学—分子生物学]

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1Bartei, D.E (2009). MicroRNAs: target recognition and regulatory functions. Cell 136: 215-233.
2Borsani, O., Zhu, J., Verslues, P.E., Sunkar, R., and Zhu, J,K. (2005). Endogenous siRNAs derived from a pair of natural cis-antisense transcripts regulate salt tolerance in Arabidopsis. Cell 123: 1279-1291.
3Brown, J.R., and Doolittle, W.F. (1995). Root of the universal tree of life based on ancient aminoacyl-tRNA synthetase gene duplications. Proc. Natl. Acad. Sci. USA 92: 2441-2445.
4Capodici, d., Kariko, K., and Weissman, D. (2002). Inhibition of HIV-1 infection by small interfering RNA-mediated RNA interference. J. Immunol. 169: 5196-5201.
5Carmell, M.A., and Hannon, G.J. (2004). RNase III enzymes and the initiation of gene silencing. Nat. Struct. Mol. Biol. 11: 214-218.
6Carmell, M.A., Xuan, Z., Zhang, M.Q., and Hannon, G.J. (2002). The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis. Genes Dev. 16: 2733-2742.
7Crouch, R.J., Arudchandran, A., and Cerritelli, S.M. (2001). RNase H1 of Saccharomyces cerevisiae: methods and nomenclature. Methods Enzymol. 341: 395-413.
8Eddy, S.R. (2001). Non-coding RNA genes and the modern RNA world. Nat. Rev. Genet. 2: 919-929.
9Edgar, R.C. (2004a). MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 32: 1792-1797.
10Edgar, R.C. (2004b). MUSCLE: a multiple sequence alignment method with reduced time and space complexity. BMC Bioinformatics 5: 113-131.