冰冻干燥G6PD反应试剂的制备及应用

Preparation of Freeze-dried Mixed Reagent for G6PD Fluorescent Spot Test and Its Clinical Application

根据葡萄糖一个磷酸脱氢酶（G6PD）荧光斑点试验方法，制备冰冻干燥G6PD反应试剂，观察冰冻干燥试剂在保存中的稳定性，用该试剂定性测定正常人和挑N缺乏者酶活性，并与G6血活性定量测定结果比较。结果冻干G6PD反应试剂在4℃至少能稳定6mo。与G6PD活性直接测速结果比较，检测G6PD活性正常者标本，符合率为92％～96％，男性G6PD缺乏者，符合率为100％。G6PD反应试剂经冰冻干燥后有利于贮存，且不影响试剂的检测效果。有条件的实验室可以批量生产，并可提供给基层医疗单位开展G6PD缺乏症的筛选。

Mixed reagent for the glucose- 6-phosphate dehydrogenase (G6PD) fluorescent spot test were freeze -dried in plastic tubes and stored at 4 ℃. The stability of the freeze-dried mixed reagents was studied. The activity of G6PD from normal and G6PD deficient blood samples was tested according to the method of fluorescent spot test with the reagent, and the results were compared with those of spectrophotometric assay. The results indicated that freeze-dried mixed reagents remained stable for at least 6 months at 4 ℃. The coincidence rate of the results of simultaneous determination of normal blood samples of both sexes, or G6PD deficient blood samples from males by means of both methods was 92%~ 96% and 100% respectively. Freeze - dried mixed reagents can be kept at 4 ℃ and don't required to be stored at meov20. A major laboratory with freeze-drying facilities is able to prepare these reagent tubes in bulk and supply small laboratories for screening purposes.