血清α-L-岩藻糖苷酶动力学测定研究

The Dynamic Determination for Serum α-L-Fucosidase

目的：建立一种简便、快捷、准确的血清α-L-岩藻糖苷酶检测方法。方法；报道了血清α-L-岩藻糖苷酶动力学连续监测法。结果：α-L-岩藻糖苷酶对硝基苯α-L-岩藻吡喃喝糖苷的米氏常数为0．133±0．052mmol／L，最适pH为5．0，延迟时间30s，线性反应时间从60～180s，批内CV5．2％，批间CV6．99％。该法基本解决了血清黄疸与微量溶血等因数对测定结果的影响。新疆地区的α-L-岩藻糖苷酶活性正常参考值范围为96．25±81．8nkat／L（2s），肝癌患者AFU阳性率为76．0％（19／25）。结论：研究结果表明，该法具有简单、快速、灵敏度高、准确等优点，适合于自动化检测和临床应用。

Objective To establish a simple , rapid and accurate serumα - L -Fucosidase (AFU)testing method.Methods A continuous monitoring method of the Dynamic Determination for Serum AFU. Result P-nitropheny α-L-Fuco-pyranoside the Km's is the 0. 133±0.052 mmol/L. The most appropriate PH is 5. 0 and the delay time was 30 seconds. The time of linear reaction lasts from 60 seconds to 180 seconds. The intra-assay CV is 5. 2% and inter-assay CV is 6. 99 %. The method basically solves the affection to the testing results from the factor such as serum jaundice and micro - haemolysis. The reference HFU value of normal person in Xinjiang area is 96. 25±81. 8 nkat/L (2s). The AFU positive rate of hepatocellular carcinoma is 76. 0% (19/25). Conclusion The result shows that the method is rapid, simple, accurate, and sensitive. It is suitable for the automatic assay and clinical application.