软骨抗血管生成组份的分离纯化及性质研究

Study on the Purification of Angiogenesis Inhibitor from Shark Cartilage and Its Biological Characteristics

目的从鲨鱼软骨中分离纯化血管生成抑制剂（CDAI）并对其性质加以研究。方法2MNaCl抽提；CM－SephadexC－50离子交换层析；DEAE－SephadexA－50离子交换层析；SephadexG－50凝胶层析。SDS一聚丙烯酰胺凝胶电泳纯度鉴定。MTT法测定CDAI对1H11内皮细胞的作用。鸡胚绒毛尿囊膜试验（CAM）观察CDAI对血管生成的作用。结果得到电泳纯的CDAI，分子量为36280，CDAI对内皮细胞产生强的抑制作用，0．65mg／L时对1H11内皮细胞的增殖抑制率为50％。鸡胚绒毛尿囊膜试验显示CDAI对血管形成有显著的抑制作用。结论鲨鱼软骨中含有血管生成抑制剂，能抑制血管生长，对具有病态血管形成的疾病具有潜在治疗价值。

Objective Cartilage -derived angiogenesis inhibitor was purified from shark cartilage and its biological characteristics were studied .Methods ①Extraction from 2M NaCI ②CM -Sephadex C- 50 ion exchange chro matographay③ion exchange on a DEAE-Sephades A-50 column ④gel filtration on a Sephadex G-50 column⑤SDS polyacrylamide gel electrophoresis(PAGE)⑥MTT colorimetric assay used to determine the effects of CDAl on the memtion of IH11 cells and some tumor cell lines ⑦ the effects of CDAI on neovascularization observed by chorioallantoic mem brane (CAM) test. Results CDAl appeared as single band of apparently related molecular mass (Mr) of 36 280 as determined by SDS - PAGE analysis. CDAI can strongly inhibit the proliferation of 1H11 cells and the angiogenesis in the chick chorioallantoic memebrane.Conchusion Shark cartilages contain the inhibitors of angiogenesis and can inhibit neovascularization so they have therapeutic value to those diseases with pathological angiogenesis.[