摘要
目的观察脑源性神经营养因子（ＢＤＮＦ）基因工程成肌细胞脑内纹状体移植对帕金森病（ＰＤ）大鼠脑内纹状体多巴胺含量的影响。方法建立逆转录病毒介导的ＢＤＮＦ表达质粒，并转染成肌细胞进行ＰＤ大鼠脑内纹状体移植。结果细胞移植后第２和第８周，移植组毁损侧纹状体多巴胺含量〔分别为（９５７５３±８８９５）和（１０４０２９±１０４７８）ｐｇ／ｍｇ〕较对照组〔分别为（３３５９８±１０２４８）和（３２７８８±７０２３）ｐｇ／ｍｇ〕明显增加（均为Ｐ＜００１），并可维持２个月之久。结论脑源性神经营养因子基因工程成肌细胞脑内纹状体移植，可使脑内纹状体多巴胺含量明显增加，可能为帕金森病的治疗提供了一种新的治疗方法。
Objective To observe the role of
genetically engineered myoblast on Parkinson's rat models. Methods Genetically modified
myoblasts capable of producing brain derived neurotrophic factor (BDNF) were grafted into the
striatum of Parkinson's rats and the content of dopamine in the striatum was detected with
highperformance liquid chromatography (HPLC) after transplantation at different time. Results
In the graft group, the dopamine content in the striatum on the lesioned side increased
significantly as compared with that in the control group (957.53 to 335.98 pg/mg in week 2 and
1 040.29 to 327.88 pg/mg in week 8, P<0.01 in both). Conclusions BDNF secreting myoblasts
may promote the survival of neuron and may proivde a new approach to the treatment of
Parkinson's disease.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
1999年第3期167-169,共3页
Chinese Journal of Geriatrics
关键词
帕金森病
多巴胺
基因疗法
BDNF
short chain fatty acids
total parenteral nutrition
morphology
proliferation indexParkinson disease Brainderived neurotrophic
factorDopamineGene therapy
