人Ⅱ型免疫缺陷病毒gag基因在大肠杆菌中的表达

Expression of the HIV2 gag gene in E.coli

目的：表达ＨＩＶ２型基因工程ｇａｇ抗原。方法：将编码ＨＩＶ２型ｇａｇ的部分和全部ｐ５５ｇａｇ１／２基因，克隆到载体ｐＥＴ１７ｂ后，分别在Ｅ．ＣｏｌｉＢＬ２１（ＤＥ３）中表达。结果：表达产物为５１和６１ｋＤ融合蛋白，并可与ＨＩＶ２病人血清发生特异性反应。表达量分别为菌体总蛋白的１２．２％和６．２％。结论：上述两种ｇａｇ重组蛋白可在Ｅ．Ｃｏｌｉ中得到表达，且有良好的抗原性。

Objective: To express HIV2 gag protein in E.coli. Methods: Insert the entire and truncated gag gene into the expressing vector pET17b respectively,then transform and culture E.coli BL21(DE3). Results :Expressed fusion proteins of 51,61 kD could be recognized by serum from HIV2 infected patients. They occupied 12.2% and 6.2% of the expressing host's total proteins respectively. Conclusion: The HIV2 gag recombinant protein could be expressed in E.coli. The expressed gag protein has perfect antigenicity.