胸腺内接种同种脾细胞诱导新生期大鼠免疫耐受的研究

Immunologic tolerance induced by neonatal intrathymic injection of allo-geneic spleen cells

为了诱导新生大鼠免疫耐受。方法：将 ２．５ ｘ １０７个 Ｗｉｓｔａｒ大鼠脾淋巴细胞通过胸腺途径注射给 新生ＳＤ大鼠，４－６周龄时取其脾淋巴细胞与Ｗｉｓｔａｒ大鼠脾淋巴细胞（２０ Ｇｙ照射）进行混合培养，作氚标记并测定 放射强度。结果：实验组放射强度平均为（１０．８５±２．５６）Ｂｑ；对照组为（２４．８２±５．４４）Ｂｑ，２组比较差异显著（Ｐ＜ ０．００１）。结论：新生期大鼠胸腺内接种同种脾淋巴细胞可以诱导其免疫耐受。

Aim:To induce immunologic tolerance in neonatal SD rat.Methods:Intrathymic injection of 2.5 10 7Wistar rat spleen cells in neonates was followed by mixed lymphocyte culture(MLC)with Wistar rat spleen cells(20 Gy)qt 4~6weeks of age.Results:The value of Bq in neonatal primed rats is Significantly lower than that in control rats(10.85 2.56)Bq and (24.82 5.44)Bq respectively(P<0.001).conclusion:Intrathymic inoculation of allogeneic cells in neonatal rats with immature immune system can produe immunologic tolerance in vitro.