摘要
观察葛根总黄酮的毒性。方法:采用简化机率单位法计算其对小鼠的急性毒性(LD50)。结果:腹 腔注射LD50为5.97(5.46-6.48)g/kg,经口灌胃LD50为10.11(9.41-10.8)g/kg。对大鼠长期毒性:给动物经口灌胃 葛根总黄酮剂量为 2.50,1.66及0.83 g/kg,1次/d,连用90 d;动物一般情况良好,除大、中剂量对雄性动物体重及白 细胞总数稍有影响外,对血小板、血色素、肝肾功能及各主要脏器病理组织学均无明显影响。结论:长期应用本品 可能不产生蓄积性毒性。
Aim:To observe acute and long-term toxicity of crude flavone of pueraria lobata(CFPL)in mice and rats.Methods: The experiments were designed for determing LD_50 dose of CFPL in mice .LD50and 95% confidence limit were determined with simplify probit unit method.The test was designed to determine long-term toxic effects of CFPL at the dose of 2.50,1.66, 0.83g/(kg·d) ig for 90 days in rats.Results:The LD_50 was 5.97(5.46~6.48)g/kg when CFPL was given ip,and 10.11(9.41~10.81)g/kg when given ig.The results of long -term toxicity showed that during the whole experimental period,the general condition of rats were good;Leucocyte and body weight of animals in the groups of large and middle doses decreased a little and returned to normal 15 days after stopping treatment.No noticeable toxicity was found in the kidney and liver function test,histopathological examination, platelet and red cell and hemoglobin.Conclusion:The CFPL has no accumulated toxic effect in rats.
出处
《河南医科大学学报》
1999年第2期48-50,共3页
Journal of Henan Medical University