葛根总黄酮的急性毒性及长期毒性实验

Study on acute and long-term toxicity of crude flovone of pueraria lobata

观察葛根总黄酮的毒性。方法：采用简化机率单位法计算其对小鼠的急性毒性（ＬＤ５０）。结果：腹 腔注射ＬＤ５０为５．９７（５．４６－６．４８）ｇ／ｋｇ，经口灌胃ＬＤ５０为１０．１１（９．４１－１０．８）ｇ／ｋｇ。对大鼠长期毒性：给动物经口灌胃 葛根总黄酮剂量为 ２．５０，１．６６及０．８３ ｇ／ｋｇ，１次／ｄ，连用９０ ｄ；动物一般情况良好，除大、中剂量对雄性动物体重及白 细胞总数稍有影响外，对血小板、血色素、肝肾功能及各主要脏器病理组织学均无明显影响。结论：长期应用本品 可能不产生蓄积性毒性。

Aim:To observe acute and long-term toxicity of crude flavone of pueraria lobata(CFPL)in mice and rats.Methods: The experiments were designed for determing LD_50 dose of CFPL in mice .LD50and 95% confidence limit were determined with simplify probit unit method.The test was designed to determine long-term toxic effects of CFPL at the dose of 2.50,1.66, 0.83g/(kg·d) ig for 90 days in rats.Results:The LD_50 was 5.97(5.46~6.48)g/kg when CFPL was given ip,and 10.11(9.41~10.81)g/kg when given ig.The results of long -term toxicity showed that during the whole experimental period,the general condition of rats were good;Leucocyte and body weight of animals in the groups of large and middle doses decreased a little and returned to normal 15 days after stopping treatment.No noticeable toxicity was found in the kidney and liver function test,histopathological examination, platelet and red cell and hemoglobin.Conclusion:The CFPL has no accumulated toxic effect in rats.