绿原酸在家兔体内的药动学研究

Study on Pharmacokinetics of Chlorogenic Acid in Rabbits

建立了血浆中绿原酸的HPLC分析方法,比较了金银花三种注射液中绿原酸在家兔体内的药动学差异,旨在为研究金银花连翘药提供实验数据。将18只健康无伤的家兔随机分成三组,按0.5 mL/kg单次肌肉注射给药,HPLC同时测定不同时间点血浆中绿原酸浓度。用MCPKP程序计算动力学参数。健康家兔肌注金银花单提注射液(A),金银花、连翘混合提取注射液(B),金银花、连翘单提合并注射液(C)后,绿原酸在家兔体内的药动学过程均符合一级吸收二室模型。B、C注射液中绿原酸的Tmax、Tα/2、T1/2ka与A相比呈差异显著性,而Tβ/2差异不显著,表明金银花配伍连翘后能促进绿原酸在体内吸收分布,迅速达到血峰浓度,对消除半衰期的影响不大。B与C中绿原酸的药动学参数差异不显著,表明连翘与金银花配伍时,不管是混提还是单提后合并,对绿原酸在家兔体内的药动学特征无明显影响。

In this article,we set up a method to analyse chlorogenic acid in plasma by HPLC,compare the pharmacokinetic difference of chlorogenic acid in three kinds of honeysuckle injection in rabbits in order to analysis materia compoent of honeysuckle and forsythiae.18 health rabbits which injected with 0.5 mL/kg in muscle are divided into three groups,and determined chlorogenic acid concentrations in plasma at different time points by HPLC.The pharmacokinetic parameters are calculated by MCPKP program.The pharmacokinetic process of chlorogenic acid in rabbits is two-compartment model.The parameter of Tmax,Tα/2,T1/2ka have significant difference between B,C and A,Tβ/2 has no significance,which show that chlorogenic acid can soon distribute in the body.The parameter of B and C have no significance,it is also show that forsythia can promote the chlorogenic acid＇s absorption and distribution in rabbit.There is no significant difference in chlorogenic acid pharmacokinetic process between B and C.