反相高效液相色谱法测定硝碘酚腈含量

Quantity Study of Nitroxynil by RP-HPLC

采用反相高效液相色谱法(RP-HPLC)测定了硝碘酚腈含量。以乙腈-0.01 mol/L磷酸盐pH6.5(20∶80,V/V)为流动相,Agilent Zorbax SB-C18色谱柱(4.6 mm×250 mm,5μm),流速1.0 mL/min,柱温25℃,检测波长273 nm。结果表明,在0.050～1.20 mg/mL范围内硝碘酚腈浓度与峰面积呈良好线性关系(r=0.9995),最低检出浓度为2.5 ng/mL,最低定量浓度为5.0 ng/mL。低、中、高3种浓度回收率分别为100.21%、99.07%、101.90%,RSD=0.48%(n=9)。本方法专属性强,准确、简便、灵敏度高,结果稳定,可用于硝碘酚腈的含量测定。

Nitroxynil was determined by a RP-HPLC method.The method was performed with a Agilent Zorbax SB-C18 column（4.6 mm×250 mm,5 μm） at 25 ℃,and the mobile phase was acetonitrile-0.01 mol/L phosphate pH6.5（20∶80,V/V）.The flow rate was 1.0 mL/min.The detection wavelength was 273 nm.The result showed that there was a good linear relationship（r=0.999 5） between peak area and nitroxynil concentration at the range of 0.050～1.20 mg/mL,the limit of detection was 2.5 ng/mL,the limit of quantitation was 5.0 ng/mL.The recovery for low,medium and high concentration was 100.21%,99.07% and 101.90% respectively,RSD was 0.48%（n=9）.The method is selective and simple that suitable for assay of nitroxynil sample.