摘要
本实验研究利用细粒棘球蚴(Echinococcusgranulosus)囊内容物及其提纯抗原复制过敏反应模型。将100只昆明种小白鼠,根据决定性注射致敏原的不同,随机分为羊源囊液抗原组、羊源囊液部分纯化抗原组、人源囊液抗原组和人源囊液部分纯化抗原组,并设立生理盐水对照组。以腹腔注射方式进行攻击,复制出小白鼠过敏反应模型。结果表明,四个致敏原组过敏反应发生率分别为94.4%、89.5%、95%和94.4%,而对照组则无一例发生过敏反应。同时,各组决定性注射前后分别进行血嗜酸性粒细胞直接计数,并与健康小白鼠对照,发现过敏反应发生后血中嗜酸性粒细胞计数明显升高(P<0.05)。提示该模型症状典型,方法简单。
Mice anaphyiactic models were successfully reproduced by Echinococus granulosus (E.g.)
cystic fluid and by its partially purified antigens. 100 mice with a weight of 18 ̄22g were
intraperioneally inoculated by E.g Protoscolices and resulted in echinococcosis. According to
hte challenge injections of the different sensitinogens, the animals were randomly divided into
five groups called (1) sheep fresh cystic fluid, (2) sheep partially purified cystic fluid, (3) human
fresh cystic fluid, (4) human partially purified cystic fluid group and (5) 0.9% natural solution
group as control. The anaphylactic models were reproduced in the mice by intraperitoneal
injection. The results of the four experimental groups incidences of anaphylaxis were 94.4%,
89.5%, 95% and 94.4% respectively, while none of the mice in the control groups showed
anaphylaxis. Meantime, the experimental groups were detected by direct eosinophilic leukocyte
count (DELC) in prechallenge and postchallenge. Healthy mice without challoccosis. \ \ The
results indicated DELC in blood samples increased significantly after the appearance of
anaphylaxis ( P <0.05) . This finding showed that the anaphylactic models with typical
symptoms and practical method, would have a promising application in experimental study and
the clicial of the echinocenge were also tested by DELC in comparison with the experimental
groups.
出处
《地方病通报》
1999年第2期16-20,共5页
Endemic Diseases Bulletin
基金
国家自然科学基金
关键词
细粒棘球蚴
原头节
过敏反应
动物模型
E.granulosus
Protoscolices
Hydatid disease
Anaphylaxis
Animal modils
Kunming mice