夏枯草化学成分研究

Study on Chemical Constituents of Prunella vulgaris L.

采用柱色谱分离技术,从夏枯草全草70%乙醇浸膏的乙酸乙酯和正丁醇部位中分离得到14个化合物,经MS和NMR分析鉴定为:白桦脂酸(1)、乌苏酸(2)、2α,3α-二羟基-12-烯-28-乌苏酸(3)、2α,3β-二羟基-12-烯-28-乌苏酸(4)、2α,3β,24-三羟基-12-烯-28-乌苏酸(5)、2α,3α,19-三羟基-12-烯-28-乌苏酸(6)、齐墩果酸(7)、槲皮素-3-O-β-D-葡萄糖苷(8)、槲皮素-3-O-β-D-半乳糖苷(9)、山奈酚-3-O-β-D-葡萄糖苷(10)、芦丁(11)、丹参素甲酯(12)、丹参素乙酯(13)、迷迭香酸乙酯(14)。其中化合物5、6、10、13为首次从该植物中分离得到,化合物5、13为首次从该属植物中分离得到。

Column chromatographic technique was used for the isolation and purification of the chemical constituents of the ethyl acetate portion and the n-butanol portion from 70% ethanol extract of the whole grass of Prunella vulgaris L.Fourteen compounds were identified as：betulinic acid（1）,ursolic acid（2）,2α,3α,-dihydroxy12-en-28-ursolic acid（3）,2α,3β,-dihydroxy-12-en-28-ursolic acid（4）,2α,3β,24-trihydroxy-12-en-28-ursolic acid（5）,2α,3α,19-trihydroxy-12-en-28-ursolic acid（6）,oleanolic acid（7）,quercetin-3-O-β-D-glucoside（8）,quercetin-3-Oβ-D-galactoside（9）,kaempferol-3-O-β-D-glucoside（10）,rutin（11）,methyl 3,4-dihydroxy-phenyl lactate（12）,ethyl 3,4-dihydroxyphenyl lactate（13）,ethyl rosmarinate（14） by means of MS and NMR spectroscopy.Among them,compounds 5,6,10 and 13 were first isolated from Prunella vulgaris L.,and compound 5 and 13 were have been found in Prunella for the first time.