水稻黑条矮缩病抗性资源的筛选和抗性QTL的定位

Screening of Rice Resources against Rice Black-Streaked Dwarf Virus and Mapping of Resistant QTL

通过水稻黑条矮缩病的田间鉴定发现,分蘖盛期的病状最为显著,是病症观察的最佳时期。对江苏省311份粳稻品种进行重病区田间鉴定试验,未发现对黑条矮缩病毒(RBSDV)免疫的品种。江苏省目前正在推广的24个主栽水稻品种发病率在10.0%～30.0%之间,曾推广的287份粳稻品种中,71.5%的品种发病率在10.0%～29.0%之间,其余品种发病率在29%以上。来源于日本的粳稻品种Koshihikari的黑条矮缩病发病率相对较低,籼稻高产品种桂朝2号为感病品种。利用Koshihikari/桂朝2号RILs群体进行抗RBSDV的基因定位,结果在第3染色体上标记RM7～RM5748之间检测到1个抗黑条矮缩病的QTL,来自Koshihikari的等位基因增强了水稻黑条矮缩病的抗性,携带抗性位点的家系明显提高了对RBSDV的抗性。本研究结果将为研究水稻黑条矮缩病和水稻抗黑条矮缩病分子育种提供重要信息。

Rice black-streaked dwarf virus（RBSDV） disease become epidemic in Jiangsu and Zhejiang provinces.To screen resistant germplasms and discover new resistance genes/QTLs against RBSDV,we evaluated the resistance to RBSDV in 311 japonica varieties in field test.The results showed that no variety was immune to RBSDV.The disease rate of 24 main japonica varieties grown in Jiangsu province at present was 10.0%–29.0% and that of 71.5% varieties in 287 japonica varieties popularized in Jiangsu before was between 10.0% and 30.0%.Furthermore,quantitative trait loci（QTL） analysis was conducted by using 162 recombinant inbred lines（RILs）,which derived from a cross between Guichao 2,a susceptible indica variety,and Koshihikari,a japonica variety with resistance to RBSDV.RBSDV resistances were evaluated using natural infection methods by scoring the disease rating.One putative QTL（qRBSDV3） controlling RBSDV resistance was mapped between the marker RM7 and RM5748 on chromosome 3,which explained 17.1% of the total phenotypic variation with LOD score of 5.4.The positive resistant effect contributed from Koshihikari.Further analysis revealed that the lines harboring the alleles of qRBSDV3 exhibited significantly increased resistance to RBSDV.The results should be very useful for breeding new RBSDV-resistant cultivars by marker-assisted selection（MAS）.