利用RIL群体创造抗黄曲霉兼抗青枯病的高油花生新种质

Novel High Oil Germplasm with Resistance to Aspergillus flavus and Bacterial Wilt Developed from Recombinant Inbred Lines

协同提高抗青枯病花生品种的黄曲霉抗性及含油量是我国花生育种的重要目标之一。利用远杂9102×中花5号杂交后代衍生的重组近交系群体(RIL),通过黄曲霉抗性、青枯病抗性鉴定及含油量测试,表明黄曲霉抗性受2对连锁并具累加作用主基因+加性多基因控制,含油量受2对具抑制作用主基因+加性多基因控制,RIL群体的黄曲霉抗性和含油量变异远远超过双亲的差异,表明它们均具有通过互补产生超亲性状的潜力。获得了抗黄曲霉或抗青枯病的高油后代家系18份,其中抗黄曲霉兼抗青枯病高油新种质1份(J091)。农艺性状和SSR分析结果表明,18份后代材料具丰富的遗传多样性,农艺性状优良,具有重要育种价值。

One of the important goals for peanut breeding in China is to enhance the resistance to aflatoxin accumulation and bacterial wilt（Ralstonia solanacearum） as well as to increase the oil content.In the present study,recombinant inbred lines were constructed with the offspring derived from Yuanza 9120×Zhonghua 5.Yuanza 9120 with moderate oil content has high resistance to bacterial wilt but no resistance to aflatoxin accumulation;Zhonghua 5 is high yielding,high susceptible to bacterial wilt and not resistant to aflatoxin accumulation.Resistances to aflatoxining and bacterial wilt were identified in F7-10 as well as the oil content and agronomic traits of lines were investigated.The results indicated that resistance to Aspergillus flavus was controlled accumulatively by two linked main genes and other additive genes,and oil content was controlled by two inhibitory main genes as well as other additive genes.There were significant difference for bacterial wilt resistance,resistance to Aspergillus flavus and oil content between the offspring and parents which presented heterobeltiosis.Eighteen high oil content lines with resistance to to Aspergillus flavus or bacterial wilt were obtained,one（J091） of which was resistant both to Aspergillus flavus and bacterial wilt.Analysis of agronomic traits and SSR data indicated that 18 lines were considerably diverse and may be used as potential breeding materials.