AA818342等6个新基因参与大鼠再生肝8种细胞的PLC信号转导

AA818342 et al Six New Genes are Involved in PLC Signaling Pathway in Rat 8 Kinds of Regenerating Liver Cells

为了解AA818342、BM389035、BF289002、BF403759、AI170687、AI715484等6个新基因在PLC信号通路中的作用及与大鼠肝再生的相关性,分离大鼠再生肝8种细胞,检测它们的基因表达变化,分析新基因与已知基因的序列同源性、共表达关系及参与的生理活动.结果表明,AA818342与col8a1同源,在30 h和72 h再生肝的卵圆细胞中表达下调,在各期再生肝的库普弗细胞中表达上调.BM389035与itga1同源,在168 h再生肝的树突状细胞中表达下调.BF289002与gnai1同源,在12 h和168 h再生肝的卵圆细胞表达上调.BF403759与cacna1d同源,在2 h再生肝的星形细胞表达上调.AI170687与mef2c同源,在36 h再生肝的树突状细胞中表达下调.AI715484与prkce同源,在2 h再生肝的星形细胞中表达上调.上述基因转录谱预示,AA818342等6个新基因属于PLC信号通路成分,参与大鼠再生肝8种细胞的PLC信号转导.

To explore the roles of 6 new genes including AA818342,BM389035,BF289002,BF403759,AI170687 and AI715484 in PLC signaling pathway and the relevance of them with rat liver regeneration,in this investigation,isolation of rat 8 kinds of liver cells and detection of genes expression changes were performed,and the new genes and their known homology and co-expression relation and the possible physiological activities were analyzed according to the method described by Zhang et al[1].It was found that AA818342 and col8a1 were homologous and down-regulation in oval cell at 30 and 72 h after partial hepatectomy,while up-regulation in Kupffer cell during the whole liver regeneration.BM389035 and itga1 were homologous and down-regulation in dendritic cell at 168 h.BF289002 and gnai1 were homologous and up-regulation in oval cell at 12 and 168 h.BF403759 and cacna1d were homologous and up-regulation in hepatic stellate cell at 2 h.AI170687 and mef2c were homologous and down-regulation in dendritic cell at 36 h.AI715484 and prkce were homologous and upregulation in hepatic stellate cell at 2 h.According to above gene transcription profiles,it inferred that AA818342 et al 6 new genes were the components of PLC signaling pathway and were possibly involved in PLC signaling cascade in rat 8 kinds of regenerating liver cells.