不孕症42例的TORCH、CBV联合检测

Combined detection of toxoplasma rubella cytomegalic virus and coxsackie B vrius in 42 sterility patients

目的：探讨ＴＯＲＣＨ（弓形体、风疹病毒和巨细胞病毒的合称）、ＣＢＶ（柯萨奇Ｂ组病毒）感染与不孕症之间的关系。方法：采用抗体捕获酶联免疫和间接酶联免疫法技术，检测４２例不孕症患者血清中ＴＯＲＣＨ、ＣＢＶ特异性抗体ＩｇＭ、ｌｇＧ，并与９２例早孕妇女、２６例健康经产妇、２８例晚孕妇女相对照。结果：（１）不孕症患者ＴＯＲＣＨ总感染率（２３８％）与孕妇组总感染率（５４％），经统计学处理，差别非常显著（Ｐ＜００２）。其中，风疹病毒感染率不孕症组（９５％）与孕妇组感染率（１０８％）比较，存在显著性差异（Ｐ＜００５）；不孕症组弓形体和巨细胞病毒感染率分别为９５％、４７６％，与孕妇组（３６％、１０８％）比较，差别无显著性（Ｐ＞００５）。（２）不孕症组ＣＢＶ－ＩｇＭ感染率（６４３％）与健康经产妇感染率（１５％），经统计学处理，差别非常显著（Ｐ＜０００１）；与孕妇组（３７５％）比较，差别有显著性（Ｐ＜００５）。（３）不孕症组存在两种病原体混合感染情况。结论：提示ＴＯＲＣＨ、ＣＢＶ感染是不孕症的重要病因之一。

Objective:To Study the relationship between TORCH,CBV and sterility.Methods:Capture Enzyme Linked Immunosobent Assay(CELISA)and Indirect Enzyme Linked Immunosobent Assay(IELISA)technique was employed to detect TORCH,CBV specific antibodies(IgM,IgG) in the serum of 42 sterile patients,which were compared with 92 early pregnant women,26 health multigravidas ,28 late pregnant women Results:(1)The overall TORCH infection rate was 23 8% in sterile patients and 5 4% in pregnant women,there were very significant difference between them by the process of statistics(P<0 02).The rubella infection rate was 9 5% in sterile patients,which was significantly different from 1 08% in pregnant women(P<0 05).The toxoplasma and cytomegalic infection rate were 9 5% and 4 76% in sterile patients respectively,which were not significantly different from 3 6% and 1 08% in pregnant women respectively(P>0 05).(2)The CBV IgM infection rate of 64 3% in sterile patients which was more significantly different from 15% in health multigravidas (P<0 001)by the process of statistics maked a significant difference from 37 5% in pregnant women(P<0 05).(3)There were examples of two pathogene mixed infection in sterile patients .Conclusions:It is suggested that the TORCH and CBV infection is one of the important causes of sterility.