基于泡沫分馏技术立体选择拆分蛋氨酸外消旋体

Enantioselective Resolution of Racemic Methionine Based on Bubble Fractionation

以青霉素G钾盐(PEN-K)为手性捕集剂,十二烷基硫酸钠(SDS)为起泡剂,采用泡沫分馏技术对蛋氨酸外消旋体进行拆分,采用正交实验法考察了回流时间(A)、溶液pH值(B)、柱填料高度(C)、回流气速(D)和待分馏溶液中各物质浓度比(E)等对拆分性能的影响。通过直观分析和方差分析研究可知,五因素中对对映体过量值影响次序为:D>E>B>A>C,从而得到拆分蛋氨酸外消旋体的优化工艺条件为D2E2B1A4C4。在回流时间60min、溶液pH值2.0、柱填料高度28cm、回流气速40L/h、溶液中各物质浓度比60∶1∶0.6的条件下得到的对映体过量值为50.0。

A method of bubble fractionation,using benzylpenicillin potassium （PEN-K） as a chiral collector and sodium dodecyl sulfate （SDS） as a foamer,was developed for the resolution of racemic methionine （racMet）. Orthogonal experiment was performed in the research of enantioselectivity of Met,the influence of reflux equilibration time （A）,pH of solution （B）,packing height （C）,gas flow rate （D） and concentration ratio of different substances in solution on resolution performances were investigated. It is found with intuitionistic analysis and variance analysis that the influence sequence in five factors on enantioselectivity is DEBAC,and thus the optimal conditions of process were obtained from orthogonal test data as follows：D2E2B1A4C4,and the enantiomeric excess value was 50.0 under the conditions of reflux equilibration time of 60 min,pH of solution of 2.0,packing height of 28 cm,gas flow rate of 40 L/h and concentration ratio of 60：1：0.6.