拟南芥ERECTA基因的克隆及其对番茄转化

Arabidopsis ERECTA Gene Cloning and Transferring to Tomato

全球每年因干旱而造成的作物减产和农业产量损失不可计量,因此从分子生物学上探索植物潜在的耐旱机理对于提高作物抗旱能力具有非凡意义。拟南芥(Ler型)ERECTA基因编码的蛋白存在两个不同的跨膜受体蛋白激酶。通过RT-PCR的方法从拟南芥基因中克隆出ERECTA基因,构建真核表达载体pBin438-ERECTA,转化到番茄中,为后续研究其对植物水分的利用效率奠定基础。

Drought is one of the most important environmental constraints limiting plant growth and agricultural productivity,so it is important to understand the underlying mechanism of drought tolerance and to identify genes for improving this important trait.ERECTA gene encodes a protein containing 976aa.The fragment of ERECTA gene was cloned from Arabidopsis by RT-PCR.A plant expressing vector pBin438-ERECTA was constructed.Leaf segments of tomato were inoculated by Agrobacterium tumefaciens GV3101 with plasmid pBin438-ERECTA,and PCR analysis of kanamycin resistant plants showed that the gene had been integrated into the genome of the tomato.This study could provide the foundation for the utilization of plant water.