期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

检测猪繁殖与呼吸综合征病毒RT-PCR的建立及应用 被引量：4

Establishment of RT-PCR for Detection of Porcine Reproductive and Respiratory Syndrome Virus and Its Application
下载PDF
导出
摘要 根据GenBank上PRRSV的保守序列,设计1对引物,建立了PRRSV的RT-PCR方法。试验结果表明,该RT-PCR方法敏感、特异,适于临床样品检测,为PRRSV的临床诊断和流行病学调查奠定了基础。 According to the conserved sequence of PRRSV published in GenBank,a pair of primers for amplification PRRSV specified fragment was designed and a PCR assay for detection PCV-2 was established.The results showed that the PCR was sensitive and specific,and useful to detect the clinic samples.It is the foundation for clinical diagnosis and epidemiologic survey of PRRSV.
作者 赵朴 郑玉姝 贾贝贝 刘俊伟 陈金山 姚四新 赵坤 李任峰 王三虎 刘兴友 赵恒章
机构地区 河南科技学院动物科学学院
出处 《生物技术通报》 CAS CSCD 北大核心 2010年第8期180-182,共3页 Biotechnology Bulletin
关键词 猪繁殖与呼吸综合征病毒 RT-PCR 临床诊断 流行病学调查 Porcine reproductive and respiratory syndrome virus RT-PCR Clinical diagnosis Epidemiologic survey
分类号 S858.28 [农业科学—临床兽医学]
  • 相关文献

参考文献8

  • 1Mulupuri P,Zimmerman JJ,Hermann J,et al.Antigen-specific B-cell responses to porcine reproductive and respiratory syndrome virus infection.J Virol,2008,82(1):358-370.
  • 2Costers S,Lefebvre DJ,Delputte PL,et al.Porcine reproductive and respiratory syndrome virus modulates apoptosis during replication in alveolar macrophages.Virol,2008,153(8):1453-1465.
  • 3Drew TW.A review of evidence for immunosuppression due to porcine reproductive and respiratory syndrome virus.Vet Res,2000,31(1):27-39.
  • 4Albina E.Epidemiology of porcine reproductive and respiratory syndrome(PRRS):an overview.Vet Microbiol,1997,55(1-4):309-316.
  • 5Cho JG,Dee SA.Porcine reproductive and respiratory syndrome virus.Theriogenology,2006,66(3):655-662.
  • 6姜平,董永毅,许家荣,蔡宝祥,王立钧,倪兆朝.华东部分省市PRRS和猪伪狂犬病血清流行病学调查[J].畜牧与兽医,1999,31(6):22-23. 被引量：14
  • 7赵朴,郑玉姝,贾贝贝,乔传玲,陈化兰,刘兴友,李海燕.猪流感病毒HA基因的克隆及杆状病毒转移载体的构建[J].生物技术通报,2009,25(9):97-100. 被引量：4
  • 8Guarino H,Goyal SM,Murtaugh MP,et al.Detection of porcine reproductive and respiratory syndrome virus by reverse transcription polymerase chain reaction using different regions of the viral genome.J Vet Diagn Invest,1999,11(1):27-33.

二级参考文献6

共引文献16

同被引文献21

  • 1范明远.关于人与猪的猪2型链球菌病问题[J].疾病监测,2005,20(7):337-338. 被引量：2
  • 2Brown I H, Harris P A, McCauley J W,et al. Multiple genetic re- assortment of avian and human influenza a viruses in European pigs,resulting in the emergence of an H1N2 virus of novel geno- type[J]. J Gen Virol,1998,79(12):2947-2955.
  • 3Gutierrez-Martin C B, Rodriguez-Delgado O, Alvarez-Nistal D, et al. Simultaneous serological evidence of Actinobacillus pleuro- pneumoniae,PRRS, Aujeszky' s disease and influenza viruses in Spanish finishing pigs[J]. Res Vet Sci,2000,68(1):9-13.
  • 4Ito T,Couceiro J, Kelm S, et al. Molecular basis for the genera- tion in pigs of influenza A virus with pandemic potential[J]. J Virol, 1998,72:7367-7373.
  • 5Ma W, Gramer M,Rossow K. Isolation and genetic eharaeteriza tion of new reassortant H3N1 swine influenza virus from pigs in the midwestern United States[J]. J Virol, 2006,80 (10) :5092- 5096.
  • 6Naffakh N, van der Werf S. April 2009 : an outbreak of swine-or igin influenza A(H1N1) virus with evidence for human-to-human transmission[J]. Microbes Infect, 2009,11 (8 - 9) : 725 - 728.
  • 7Richt J A,Lager K M,Janke B H. Pathogenic and antigenic prop erties of phylogenetically distinct reassortant H3N2 swine influ- enza viruses cocirculating in the United States[J]. J Clin Micro- biol, 2003,41 (7) : 3198- 3205.
  • 8Choi E J, Lee C H, Hyun B H,et al. A survey of porcine re- productive and respiratory syndrome among wild boar popula tions in Korea[J]. J Vet Sci,2012,13(4):377-383.
  • 9Topfer A, Hoper D, Blome S, et al. Sequencing approach to analyze the role of quasi species for classical swine fever[J]. Virology,2013,438(1) :14-19.
  • 10Leifer I, Ruggli N, Blome S. Approaches to define the viral genetic basis of classical swine fever virus virulence[J]. Virolo gy,2013,438(2) :51-55.

引证文献4

二级引证文献11

生物技术通报
2010年 第8期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部