摘要
目的评价mPEG-PLGA-mPEG(PELGE)空白纳米粒的体外免疫性。方法采用定量酶联检测与PELGE纳米粒共同培养后的THP-1源巨噬细胞细胞因子IL-6、IL-1β和TNF-α的释放量。结果 9种PELGE材料所制备的纳米粒中,9号材料(PEG2000、5%、LA/GA=5:5)可以导致THP-1源巨噬细胞IL-6分泌量显著高于阴性对照组和PLGA纳米粒组。结论 9号材料可能有潜在的免疫毒性,而其余8种材料具有较好的免疫相容性。
OBJECTIVE To investigate the immunotoxicity of blank mPEG-PLGA-mPEG（PELGE） nanoparticles.METHODS ABC-ELISA methods were applied to measure the release of IL-6,IL-1β and TNF-α by THP-1 cells after the incubation with blank PELGE nanoparticles.RESULTS Among the tested 9 kinds of PELGE nanoparticles,only the No.9 nanoparticles（PEG 2000,5%,LA/GA = 5：5） could cause significant increase in the release of IL-6 factor by THP-1 cells compared with that of blank control and PLGA nanoparticles group.CONCLUSION No.9 PELGE polymer might have potential immunotoxicit,while the other 8 ones might not.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2010年第4期400-402,共3页
West China Journal of Pharmaceutical Sciences
关键词
PELGE
纳米粒
体外免疫学
PELGE nanoparticles
In vitro immunotoxicology
