荞麦花叶中芦丁调节糖脂代谢的作用及机制

Role and mechanism of rutin from buckwheat flowers and leaves on metabolism of glucose and lipids

目的观察荞麦花叶中的芦丁(RBFL)对糖尿病大鼠糖脂代谢的影响并探讨其可能的作用机制。方法采用四氧嘧啶加脂肪乳诱导大鼠糖尿病模型。糖尿病大鼠ig给予低、中、高剂量RBFL共8周,以罗格列酮为阳性对照药物,动态观察RBFL对糖尿病大鼠血糖的影响;测定血糖、血脂、血清谷丙转氨酶(ALT)、胰岛素(INS)、胰岛素抗体(INS-Ab)、肿瘤坏死因子-α(TNF-α)含量;计算肝脏系数(LI)、胰岛素敏感指数(ISI);HE染色观察肝组织形态学变化,免疫组织化学法检测肝脏TNF-α蛋白表达情况。结果模型组大鼠的血糖、血脂、胰岛素、血清及肝脏中TNF-α含量明显增加,肝细胞索结构紊乱,细胞体积增大,内见大小不等的脂滴空泡。RBFL各剂量组能不同程度地降低糖尿病大鼠的血糖、血脂、胰岛素、TNF-α含量,改善胰岛素抵抗状态和肝组织的形态结构,并呈现剂量依赖关系。结论 RBFL对糖尿病大鼠糖脂代谢紊乱及胰岛素抵抗具有一定的改善作用,可能与其减少TNF-α的生成有关。

OBJECTIVE To explore the role and mechanism of rutin from buckwheat flowers and leaves（RBFL） on metabolism of glucose and lipids in diabetic rats.METHODS The diabetic rat model was induced by fat emulsion combined with small doses of alloxan.The diabetic rats were orally treated with RBFL（L,M and H dose group for 8 weeks,and Rosiglitazone was given as a positive control drug.The effects of RBFL on the fasting blood glucose（FBG）,serum TG,TC,FFA,ALT,INS,INS-Ab,TNF-α in serum and liver were detected.The liver index（LI）,INS-Ab binding rate（IABR） and ISI were calculated,and the morphological changes of liver tissue were observed.RESULTS Compared with normal control group,FBG,serum TG,TC,FFA,ALT,INS,TNF-α,LI,IABR,ISI of diabetic rats（model group） were increased.Different dosages of RBFL could decrease the above indexes in certain degree,and could improve the insulin resistance（IR） and the morphological changes of liver tissue.The effect of RBFL was increased with dose used in the rats.CONCLUSION RBFL could regulate the metabolic disorder of glucose and lipids in diabetic rats,and could improve the IR,which might be due to the reduction of TNF-α.