林蛙皮的抗氧化性研究

Study on antioxidant activities of Rana chensinensis skin

目的研究林蛙皮的抗氧化性。方法以1,1-二苯基苦基苯肼自由基(DPPH·)清除能力作为考察指标,通过正交实验确定林蛙皮抗氧化物质的最佳提取方案,并进一步测定林蛙皮提取液的清除羟自由基、超氧阴离子的能力,综合分析林蛙皮的抗氧化能力。结果林蛙皮抗氧化物质提取的最优方案为:采用冷冻干燥的方式,用蒸馏水在50℃下提取30min,其DPPH·清除率达79.12%,且DPPH·的清除能力随浓度增加而增强;林蛙皮水提物也表现出较强的·OH清除能力,但对O2-·的清除能力较弱。结论林蛙皮水提液对DPPH·和·OH具有很好的抗氧化性,但对O2-·作用不明显。

OBJECTIVE To study antioxidant activities of Rana chensinensis skin.METHODS The antioxidants extracts of Rana chensinensis skin were determined through the orthogonal experiment of DPPH scavenging capacity as the index.Moreover,the·OH and O 2^-scavenging capacity of Rana chensinensis skin extracts were analyzed.RESULTS The optimized extracts technology for the antioxidant extracts of Rana chensinensis skin was as follows：freeze drying methods for Rana chensinensis skin with water extraction solvent for 30 min at 50 ℃.The DPPH clearance rate was 79.12%,and the scavenging ability of DPPH was enhanced with increasing concentration.Rana chensinensis skin aqueous extracts also showed strong OH scavenging ability,but O 2^-removal capability was weak.CONCLUSION Rana chensinensis skin extracts had the strongest scavenging effects on DPPH·and ·OH,but showed no obvious scavenging activity effect on O 2^-.