杜仲叶HPLC指纹图谱及成分积累规律的研究

HPLC fingerprint and accumulation rule of principal component in folium eucommiae

目的建立杜仲叶的HPLC指纹图谱,分析杜仲叶中成分积累的规律。方法采用WelchromC18色谱柱;0.05%磷酸水溶液-甲醇梯度洗脱系统;柱温25℃;检测波长220nm。结果对不同产地杜仲叶的指纹图谱进行评价,生成对照指纹图谱,确定了10个共有峰,指认了第5号色谱峰为绿原酸,是第1大色谱峰。四川地区杜仲叶中的成分积累规律为:芽开绽期含量最高,次月减小,5月出现回升,再逐渐降低,7月降至最低,然后逐渐回升延续至落叶盛期。结论所做研究可为杜仲叶的质量控制及最佳采收期的确定提供参考。

OBJECTIVE To establish HPLC fingerprint of folium eucommiae and analyze its accumulation of principal component.METHODS An RP-HPLC method was used for the determination.The separation was performed on a Welchrom C18 column,and a mobile phase of gradient elution with 0.05% phosphoric acid and methanol was employed.The column temperature was 25 ℃.The detection wavelength was 220 nm.RESULTS The fingerprint of samples from different regions were analysed to get the reference chromatogram composed of ten common peaks were analyzed.The No.5 peak was the biggest one and it was confirmed to be chlorogenic acid.The accumulation rule of principal component was analyzed in folium eucommiae from Sichuan province.The maximum content was in bud sprouting period,then reduced next month,rebounded in May,gradually decreased to the minimum point till July,then gradually rose till defoliation peak period.CONCLUSION The study is useful for the quality control and chosing optimal harvest time of folium eucommiae.