热河黄芩中4种黄酮的测定及其黄酮类成分的指纹图谱研究

Determination of 4 flavonoids and fingerprint study in Rehe Scutellaria baicalensi by HPLC

目的建立HPLC同时测定道地产区种植和野生黄芩中野黄芩苷、黄芩苷、黄芩素、汉黄芩素的含量及黄酮类成分的指纹图谱研究。方法采用Diamonsil C18色谱柱(150mm×4.6mm,5μm);乙腈和水-冰醋酸-三乙胺(100:0.4:0.1)溶剂系统梯度洗脱;检测波长280nm;流速1.0mL·min-1;进样量10μL。结果野黄芩苷、黄芩苷、黄芩素、汉黄芩素的线性范围分别为2.50～80.00、10.78～345、3.50～112.0、2.00～64.00μg·mL-1(r分别为0.9999、0.9999、0.9999、0.9998);平均回收率分别为99.1%(RSD=1.7%)、100.4%(RSD=1.5%)、99.2%(RSD=1.8%)、99.8%(RSD=2.2%);确立了道地产区河北承德热河黄芩中黄酮类成分的指纹图谱有12个共有峰。结论所建方法重复性好、专属性强,可为热河黄芩药材的质量控制提供依据。

OBJECTIVE To develop an RP-HPLC method for the quantitative analysis of baicalin,scutellarin,and establish a fingerprint of flavonoids in Rehe Scutellaria baicalensi.METHODS The methanol extract of genuine Scutellaria baicalensi was separated on a Diamonsil C18（4.6 × 150 mm,5 μm） gradient elution using acetonitrile and H2O-acetic acid-triethylamine（100：0.4：0.1） solvent system and detected at 280 nm.Flow rate was 1.0 mL·min^-1 and 10 μL was injected every time.RESULTS The linear range of scutellarin,baicalin,baicalein and wogonin were 2.50-80.00 μg·mL^-1（r = 0.9999）,10.78-345.0 μg·mL^-1（r =0.9999）,3.50-112.0 μg·mL^-1（r =0.9998）,2.00-64.00 μg·mL^-1（r =0.9998）,respectively.The average recovery rates of scutellarin,baicalin,baicalein and wogonin were 99.1%（RSD = 1.7%）,100.4%（RSD = 1.5%）,99.2%（RSD = 1.8%）,99.8%（RSD = 2.2%）,respectively.In the fingerprint,12 common peaks of flavonoids in Rehe Scutellaria baicalensi were confirmed.CONLUSION The methods can be used scientifically to evaluate the quality of Rehe Scutellaria baicalensi both qualitatively and quantitatively.