肌酸激酶B同工酶快速放射免疫测定及其临床应用

A QUICK RADIOIMMUNOASSAY FOR CREATINE KINASE BB ISOENZYME AND ITS SIGNIFICANCE IN CLINICAL PRACTICE

本文建立了血清肌酸激酶BB同工酶(CK-BB)快速放射免疫测定(RIA)方法,灵敏度0.5ng/ml,测定范围0.5～100ng/ml,方法学鉴定符合RIA要求。本实验中正常组其值为1.3±1.8ng/ml。以CK-BB大于3.0ng/ml为阳性,急性心肌梗塞(AMI)组在心肌梗塞后8h男(n=22)、女(n=12)阳性率分别为86％和83％,24h均为100％。急性脑血管意外(CVA)组在脑血管意外后第1天男(n=24)、女(n=6)阳性率分别为8％和17％,第3天分别为79％和83％。方法特异性均为100％。

A quick radioimmunoassay for creatine kinase BB isoenzyme (OK-BB) is reported. The sensitivity was 0.5 ng/ml, and the range of measurements was 0.5-100ng/ml. The serum OK-BB levels in 83 normal controls were 1.3 - 0.8 ng/ml (x±SD). Values higher than 3.0ng/ml were regarded as positive. 8 hours after acute myocardial infarction, 86% were positive in males (n=22) and 83% in females(n=12). 24 hours after infarction, 100% were positive in both sexes. One the first day after cerebrovascular accidents, 8% of males (n=24) showed positive reactions and 17 % in females (n = 6). On the 3rd day positive results occurred in 79% of males and 83% of females. The specificity of this method was 100% in either group.