3种分离流感病毒方法的比较分析

Comparative Analysis of Three Methods for Isolating Influenza Viruses

目的：为了比较流感病毒3种分离方法的敏感性及确定流感优势病毒株，1996年5月～9月，对江西南昌地区流感病毒进行了分离和鉴定研究。方法：采用细胞接种培养法、鸡胚羊膜腔接种培养法、鸡胚尿囊腔接种培养法。结果：细胞接种法分离的流感病毒株14株、鸡胚羊膜腔法得12株、鸡胚尿囊腔法得2株，共28株，其中甲1型18株、甲3型4株、乙型6株。结论：流感病毒分离中，细胞接种法与鸡胚羊膜腔接种法同样敏感。鸡胚分离培养中，应高度重视羊膜腔接种法。1996年南昌地区的优势而行珠是甲1型。

Objective:To compare the sensitivity of three methods for isolating influenza viruses and to detendne the major virus strains isolated and identified in Nanchang area, Jiangxi province from May to September, 1996. Methods: The methods of cell culture, chick embryo ndotic cavity culture and chick embryo allantoic cavity culture were used. Results: Fourteen influenza vitus strains were ob tained by the cell culture, 12 by the chick embryo ameiotic cavity culture, and only 2 by the chick em bryo allantoic cavity culture. Of all the influenza virus strains, type A1, contained 18 strains, type A, four stndns and trpe B six strains. Conclusion: The sensitivity of the cell culture and the chick ernbryo anmi otic cavity culture is almost the same in isolating influenza viruses. In the chick embryo isolating cul tures, a great attention must be paid to the anmiotic cavity culture. In 1996, the major epidemic strain in Nanchang area was type A1.