中药白芨促进角质形成细胞的游走

Rhizoma Blelillae Promotes the Migration of Keratinocyte

目的 探讨中药白芨对角质形成细胞游走的影响及其对创伤愈合的作用机制。方法 采用皮肤器官培养法 (浮游法 )培养SD小鼠皮片 ,比较观察了含不同浓度白芨的培养基对角质形成细胞游走的影响及培养时间与游走长度的关系。结果 发现白芨浓度为 2× 10 -2 mg/mL及 2× 10 -3 mg/mL时 ,角质形成细胞游走比对照组显著地快和长。含白芨组更早地显示游走迹象 ,较对照组早 4h。培养48h内 ,白芨组及对照组中培养时间与游走长度皆呈直线正相关 ,白芨组的促游走作用显著地优于对照组。结论 提示白芨有明显的促进角质形成细胞游走作用 ,这种促游走作用可能对治疗皮肤创伤早期愈合有重要影响。

Objective To investigate the effect of Rhizoma Bletillae(RB) on keratinocyte migration and its mechanism in the treatment of skin wound Methods An organ culture technique of skin was applied to cultivate the skin explants of SD mice and a comparative analysis was processed to study the effect of RB on keratinocyte migration and the relationship between duration of culture and distance of migration Results Keratinocyte migration could be significantly promoted by RB, especially at a concentration of 2 ×10 -2 mg/mL or 2×10 -3 mg/mL Compared with controls, keratinocytes migrate in RB medium with a much longer distance and earlier time (4 hours earlier) (P< 0 05), the culture time and migration distance of both RB medium and controls had a linear correlation Conclusion RB can markedly promote keratinocyte migration, which may play an important role in the early healing of skin wound