用ND-O-BSA-ELISA检测小鼠血清中抗麻风杆菌IgM抗体的研究

Study on Detecton of IgM Antibody Against Mycobacterium Leprae in Murine Sera with ND O BSA ELISA

目的 为了探讨感染麻风杆菌小鼠血清中特异性IgM抗体的变化情况。 方法 用ND O BSA ELISA检测了 5 0只未感染小鼠及 12 7只感染小鼠的IgM抗体水平。结果 感染小鼠血清中IgM抗体水平非常显著地高于未感染正常小鼠 (P <0 0 1) ;未治疗组小鼠与治疗组小鼠间差异也有非常显著性 (P <0 0 1) ;而且不同抗麻风药物及同一药物不同剂量组之间抗体水平也出现明显差异 (P <0 0 1或P <0 0 5 )。结论 感染小鼠IgM抗体水平与菌负荷一致 ,并与感染程度和实际化疗结果相关。

Objective To reveal the variation of specific IgM type antibodies in the mice infected by M leprae Methods The serum level of IgM antibody against M leprae from 50 normal mice and 127 mice infected with M leprae were detected with ND O BSA ELISA Results The serum level of IgM antibody against M leprae in infected mice was significantly higher than that in normal mice(P< 0 01) There were significant differences between untreated group of mice and treated group of mice (P< 0 01) between different drug treated groups (P< 0 01), and between different dosage groups in the same drug treated mice (P< 0 01 or P< 0 05) Conclusion The serum level of IgM antibody against M leprae in infected mice is consistent with bacterial load and related to the severity of infection and the therapeutical effects