银杏过氧化氢酶基因CAT1的克隆及表达分析

Molecular Cloning,Characterization and Expression of Catalase1 Gene from Ginkgo biloba

利用RACE技术从银杏中克隆到过氧化氢酶基因(GbCAT1)的cDNA全长。进化树分析结果表明:GbCAT1和其他物种的CAT源自于相同的祖先。Southern杂交显示:GbCAT1属于1个小的多基因家族。实时定量RT-PCR分析表明:GbCAT1在银杏的根、茎、叶和果中都有表达,在叶中的相对表达量最高,其次为果、茎和根。GbCAT1的转录受到ABA、渗透压、紫外、低温和高温胁迫的诱导。水杨酸处理下,GbCAT1相对表达量迅速降低。CAT1基因在逆境条件下的相对表达变化与环境胁迫有关。

The full-length cDNA sequence of Ginkgo biloba L.Catalase1 gene was isolated from G.biloba.Phylogenetic tree analysis revealed that GbCAT1 shared the same ancestor with other CAT.The result of Southern analysis showed that GbCAT1 gene was encoded by a small gene family in G.biloba.The expression analysis by RT-PCR showed that GbCAT1 expressed in a tissue-specific manner in G.biloba.GbCAT1 was also found to be up-regulated by the four tested abiotic stress,abscisic acid,osmotic stress,low temperature,and thermal injury.The expression of GbCAT1 was regulated to reduce by salicylic acid.These results indicate that the CAT1 has the potential to play a role in response to environmental stresses.