脑钠素在心肌顿抑中的评价研究

Evaluation of BNP on cardial stunning

目的了解血浆脑钠素(BNP)是否能够反应缺血再灌注后心肌顿抑的程度。方法①建立15min缺血再灌注损伤后心肌顿抑模型。②用电生理记录仪动态观察缺血再灌注损伤前后LVSP和LVEDP的改变。③用ELISA法测定血浆中BNP值。④电镜检测缺血再灌注损伤后心肌组织学的改变。结果①心肌经15min缺血再灌注后LVSP逐渐下降,LVEDP逐渐升高;再灌注15min时LVSP降至最低,LVEDP升至最高,然后开始逐渐恢复;再灌注90min时心肌顿抑组心功能恢复正常。对照组心功能在整个实验过程中无明显改变。②心肌经15min缺血再灌注后BNP分泌增加,再灌注15min时心肌顿抑组BNP值为336.94±37.13pg/ml,再灌注90min时心肌顿抑组BNP值恢复至基线水平。对照组BNP值在整个实验过程中无明显改变。BNP值与LVSP呈负相关,而与LVEDP呈正相关。③缺血再灌注后取心尖部缺血区心肌电镜检测显示心肌顿抑组呈可逆性细胞损伤改变,对照组心肌细胞无改变。结论血浆脑钠素能良好的反应缺血再灌注后心肌顿抑程度。

Objective To research the value of BNP responses to degree of cardial stunning.Method The experimental model cardial stunning was developed.LVSP and LVEDP were record by electrophysiological recorder before and after ischemic reperfusion.Value of BNP was measured by ELISA.Myocardial histology after ischemic reperfusion was detected in electron microscope.Results LVSP gradually decreased.LVEDP was gradually elevated 15 min after ischemia reperfusion.Value of LVSP was lowest and value of LVEDP was maxlmum on 15 min after reperfusion,then they gradually restored.Cardial funtion was completely recovered in cardial stunning group.LVSP and LVEDP of control group was not affected.Value of BNP of cardial stunning group rose 15 min after ischemic repErfusion and completely recovered on 90 min after reperfusion.Value of BNP of control group was not affected.Value of BNP was negative correlation with LVSP,and positive correlation with LVEDP.Myocardial histology of ischemic region shown reversible cell injury in cardial stunning group,but myocardial cell was normal in control group.Conclusion BNP could response to degree of cardial stunning after ischemic reperfusion.