抗生素8302-1抗肿瘤作用的实验研究

EXPERIMENTAL STUDY ON ANTITUMOURAL ACTIVITY OF THE ANTIBIOTIC 8302-1

抗生素8302-1是从灰色链丝菌-变种培养发酵液中,提取的放线菌素K的同类物。本文从体内和体外观察它的抗肿瘤作甩。结果显示体外对人红白血病K562和小鼠S180细胞有明显细胞毒作用,体内对小鼠S180腹水型有明显抗肿瘤作用,作用与药物浓度、剂量和作用时间有明显相关性。腹腔注射急性半数致死量(LD_(50))与8302-1的放线菌素K相仿。结果为进一步扩大临床前药理研究提供实验依据。

Antibiotic 8302-1 may be an analogue of aotinomycin K, whioh was isolated from the culture media of Streptomyoes griseus SP. We have investigated its antitu-moural activity in vitro and in vivo. The results showed that 8302-1 had remarkable cytostatic activity on human leukemia K562 and murine S180 in vitro. It also improved the median survival time (MST) and prolonged survival(>60 days) in ascitic 8180-bearing mice. The antitumour activity and the acute median lethal dose (LD50) after intraperitoneal injection of 8302-1 were similar to those of actinomycin K. Based on these data, we recommend further preclinical pharmacological studies of the antibiotic 8302-1.