兔抗人白蛋白免疫羧化胶乳的制备以及在大肠肿瘤筛检中的应用

Preparation of Rabbit Anti-human Immuno-carboxylate and Its Application in Screening of Colorectal Tumor

目的：研究兔抗人白蛋白免疫羧化胶乳对大肠肿瘤普查的临床意义。方法：应用致敏羧化胶乳的方法制备了兔抗人白蛋白免疫羧化胶乳试剂。用反向胶乳凝集试验检测经结肠镜检出和病理证实的２６９例大肠肿瘤和结肠炎患者以及１６１例正常人的粪微量白蛋白。结果：该试剂灵敏性高（３１２５ｎｇ／ｍｌ）；检测牛、马、羊、豚鼠等动物血清呈阴性反应；用多批次免疫羧化胶乳平行检测并在有效期内重复检测已知阳性标本，其重复性良好。结论：该试剂对大肠癌的诊断敏感性７６８％，特异性８００％，准确性７７０％。

Objective: To evaluate the effect of Rabbit anti-human albumin immuno-carboxylate in screening colorectal tumors Methods: Fecal samples of 269 cases of colorectal tumor or inflammation which were confirmed endoscopically and pathologically, and samples of 161 healthy subjects as control were tested with reverse latex agglutination for trace albumin Results: The carbooxylate latex had quite high sensitivity (3125ng/ml) There was no cross reaction found in the sera of cow, horse, sheep, guinea pig etc good repetition was achieved in the intergroup parallel tests of carboxylate latex for the positive samples The results showed that the sensitivity, specificity and accuracy of the test in detecting colorectal carcinoma were 768%, 80%and 77%respectively Conclusion: Rabbit anti-human immunocarboxylate is helpful in screening colorectal tumors