慢性缺氧小鼠心肌酶组织化学分析及超微结构观察

ENZYMOHISTOCHEMICAL AND MORPHOLOGICAL CHANGES OF MYOCARDIUM OF ANOXIA MICE

目的探讨慢性缺氧对心肌酶的含量和超微结构的改变。方法３０只小鼠随机分为３组：正常对照组，吸入１５％低氧混合气组和复方丹参注射组。实验２周后，取新鲜心肌标本，测琥珀酸脱氢酶（ＳＤＨ），三磷酸腺苷（ＡＴＰ）酶，乳酸脱氢酶（ＬＤＨ）；光镜，电镜观察心肌病理性变化。结果缺氧组ＳＤＨ，ＡＴＰ酶减少，ＬＤＨ升高，心肌间质水肿，心肌细胞线粒体肿胀，肌丝排列紊乱。复方丹参组，心肌酶和心肌超微结构与对照组相比均无显著性差异。结论缺氧导致心肌酶组织化学及超微结构的改变，复方丹参对小鼠心肌有保护作用。

Objective To investigate enzymohistochemical and morphological changes of myocardium of anoxia mice.Method Thirty mice were divided invided into into three groups,the control,the hypoxia and Danshen treated group. At two weeks after treament,sections of myocardium were made,succinic dehyrogenase (SDH),adenosine trphosphatase (ATP) and lactic dehydrogenase (LDH) were stined. Pathologic changes of myocardium were observed under light microscope and electric microscope.Result In hypoxia group,SDH and ATP decreased,while LDH increased. Mesenchyma of myocardium was edematous. Mitochondrions of myocardial cells enlarged,arrangement of filaments was irregular. There was not a significant difference between Danshen treated and control group.Conclusion Anoxia causes enzymohistochemical and morphological changes of myocardium. Danshen may prevent the myocardium from damage with anoxia.