扇贝消化腺β-1,3-葡聚糖酶的分离和性质研究

Isolation and properties of β-1,3-glucanase from digestive gland of scallop

以扇贝加工废弃物消化腺为原料,经磷酸缓冲液抽提、80%饱和度硫酸铵沉淀、DE52纤维素离子交换层析和SephadexG-100分子筛层析等步骤,获得β-1,3葡聚糖酶。经SDS-PAGE检测,扇贝消化腺β-1,3-葡聚糖酶的相对分子质量为157kDa。β-1,3-葡聚糖酶水解木耳多糖(β-1,3-葡聚糖)的最适pH为7.0,最适温度为30℃。通过Lineweaver-Burk作图,得到其米氏常数Km为13mg/mL。

The β-1,3-glucanase was isolated from digestive gland of scallop by precipitation with 80% saturation of （ NH4） 2SO4,ion-exchange on DE52 cellulose and gel filtration on Sephadex G-150.SDS-PAGE showed that β-1, 3-glucanase has a molecular mass of 157kDa.The enzyme had optimal activity at pH7.0 and 30℃.The enzyme had apparent Km values of 13mg /mL by Lineweaver-Burk plot.