日照绿茶粗多糖脱蛋白研究

Study on deproteinization of Rizhao green tea crude polysaccharides

以日照绿茶粗多糖为原料,采用蛋白质脱除率、茶多糖保留率和茶多糖含量为评价指标,研究了Sevag法、三氯乙酸法和鞣酸法对茶多糖粗提物脱蛋白的影响。实验结果表明:Sevag法茶多糖含量较高、茶多糖保留率较低且操作较复杂,鞣酸法茶多糖保留率较高、蛋白质脱除率较低,三氯乙酸法的脱蛋白效果最好。用三氯乙酸脱蛋白时,茶多糖溶液pH为4.0,蛋白脱除率为68.4%,茶多糖保留率为66.8%,茶多糖含量为72.5%。

Rizhao green tea crude polysaccharides was used as material in this study. The effects of three deproteinization methods were studied by the deproteinization rate,polysaccharides retention rate and polysaccharides content. The results showed that Sevag method had higher polysaccharides content,lower polysaccharides retention rate and the operation was complicated. Digallic acid method had higher polysaccharides retention rate and lower deproteinization rate.The most effective method was trichloroacetic acid method.As the pH value of polysaccharides solution was 4.0 adjusted by trichloroacetic acid,the results were： the deproteinization rate 68.4%,the polysaccharides retention rate 66.8% and the polysaccharides content 72.5%.