水产品及水发食品中的甲醛检测技术研究

Study on formaldehyde testing technology of aquatic products and water-risen food

目的:建立了快速准确检测水产品、水发食品中的甲醛含量的方法和技术。方法:本研究采用微波萃取法和超声波萃取法代替经典的水蒸汽蒸馏法,利用甲醛在酸性条件下与2,4-二硝基苯肼反应生成2,4-二硝基苯腙,利用高效液相色谱法测定甲醛的衍生物。结果:该方法线性关系良好,线性回归方程为Y=1120.13098x+311.61308,线性范围为0.1～10μg/mL,相关系数为0.99801,加标回收率在92.3%～110.2%,用于实际样品测定,结果满意。结论:该方法操作简单、快速、准确、灵敏、适用性强,能满足水产品、水发食品中甲醛的测定。

Objective： In order to detect formaldehyde in aquatic products and water-risen food fast and accurately, the determination method was established.Methods： Microwave extraction and ultrasonic extraction method took place of the classic steam distillation.The formaldehyde in the sample reacted with the derivation reagent,2,4-dinitrophenylhydrazine,in an acidic medium to form 2,4-dinitrophenylhydrazine,then HPLC was used in determination of formaldehyde derivatives.Results： Results showed that the method had good linear relationship. The linear regression equation was established as Y =1120.13098X ＋311.61308,with linearity range from 0.1～10μg /mL and correlation coefficient of 0.99801.The recovery range was 92.3%～110.2%.The methods for the actual samples detect got satisfactory results.Conclusion： The method is simple,rapid,accurate,sensitive and applicable for the determination of formaldehyde in aquatic products and water-risen food.