^(125)Ⅰ-尿激酶放射免疫测定法

125I UROKINASE RADIOIMMUNOASSAY

作者采用氯胺T法制备^(125)Ⅰ-尿激酶(Urokinase,UK),以之免疫新西兰兔制备UK抗血清,建立了^(125)Ⅰ-UK放射免疫测定法(RIA)。本法精密度和准确性较高,批内和批间CV均小于10％,回收率为99.4±5.0％(血清)和101.±17.4％(尿液),最低检测值0.18ng/ml。用本法测定74名正常人血清和49名正常人尿液UK浓度分别为6.37±1.28ng/ml和39.30=18.04ng/ml。

A sensitive 125I-urokinase(UK) radioimmunoassay(RIA) is described. 125I-UK was prepared by the chloramine T method. Its specific radioactivity was 14.8±105 Bq/μg. The antiserum against UK was raised by immunizing healthy New Zealand white rabbits with a mixture of UK and Freund's complete adjuvant. A titer of antiserum at 1:2000, and an affinity constant of 1. 62 ±1010L/mol, was speoifio for UK. The mean recoveries in serum and urine were 99.4±5.0%and 101.1±7.4%, respectively. The value of coefficient of variation, intraassay and inter assay were both less than 10%. The low detection limit for UK antigen was 0.18ng. The detection range was 0.38-12.16 ng/tube. Serum and urine UK levels in normal controls were 6.37 ± 1.28 ng/ml (n = 74) and 39.30 ± 18.04 ng/ml (n = 49), respectively.