黄曲霉毒素的酶联免疫测定法及其在肝癌流行病学研究中的应用评价

ELISA FOR DETECTION OF AFLATOXINS AND ITS APPLICATION IN EPIDEMIOLOGICAL STUDIES OF LIVER CANCER

作者对黄曲霉毒素(Aflatoxin,AFT)B_1和M_1(AFB_1和AFM_1)的酶联免疫测定法(ELISA),AFB_1摄入量和尿中AFM_1排出量与原发性肝癌死亡率间的关系;AFB_1-辣根过氧化物酶(AFB_1-HRP)的合成及其应用于AFB_1和AFM_1的ELISA检测进行了研究。指出ELISA对样品中AFM_1、AFB_1测定最佳范围分别为0.5～10、0.5～21ng/ml。 与薄层层析法(TLC)相比,ELISA有检出率高、重复性好及专一的特点。用C_(18) Seppak处理样品不仅有效且简便快速。用ELISA检查了220份食品和230份尿液样本,证明了AFT的摄入和排泄间有很好的正相关。提示在流行病调查中,尿中AFM_1可作为人群AFB_1摄入的指标。

This paper reports the establishment of enzyme-linked immunosorbent assay (ELISA) for aflatoxin B1(AFB1) and aflatoxin M1(AFM1). Its bast range of detection was 0.5-10ng/ml for AFM1 and 0.5-21ng/ml for AFB1. AFB1-horseradish peroxidase (AFB1-HRP) was synthesized and used as a labeled antigen in the detection of AFB1 (in food) and AFM1(in urine). The relationship between dietary intake of AFB1 and the extraction of AFM1 from urine collected in areas with different mortality rates of primary hepatocellular carcinoma was analyzed.Compareds to thin layer ohromatography methods (TLO), ELISA showed much higher detection rate, specificity and greater reproduotivity, pretreatment of sam-ples with 018 seppak cartridge was very useful in avoiding tedious extration procedures. 220 food samples and 230 urine samples underwent detection by ELISA. Results demonstrated that a good relationship between total dietary AFB1 intake and urinary excretion of AFM1 in humans. These results suggest that AFM1 in urine by ELISA could be used as an index of AFB1 exposure level of AFB1 in extensive epidemiologicfil studies.