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母子隔离应激对幼鼠癫癎易感性的影响

Effect of maternal isolation stress on epilepsy susceptibility in young rats
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摘要 目的初步探讨母子隔离应激对幼鼠癫癎易感性影响及其潜在机制。方法 60只Sprague-Daw-ley幼鼠随机分成3组:对照组、生后第2~17天每天母子隔离15min组和隔离3h组。在生后18d分别行杏仁核刺激诱导惊厥实验,灌注取脑,免疫组化检测海马GABAAα1亚单位表达情况。结果与对照组比较,每天母子隔离3h组幼鼠的体重减轻,杏仁核点燃惊厥阈值降低,完全点燃所需刺激次数减少,惊厥发作程度加重,海马CA1区GABAAα1亚单位表达减少(P〈0.05),而每天母子隔离15min组与对照组幼鼠在以上几个方面比较则差异无统计学意义(P〉0.05)。结论早期每天母子隔离3h的应激对幼鼠脑发育、癫癎易感性产生不良影响,海马GABAAα1亚单位表达减少可能作为一个重要的潜在机制。 Objective To study the effect of maternal isolation stress on the epilepsy susceptibility in young rats and the possible mechanism.Methods Sixty Sprague-Dawley young rats were randomly divided into a normal control and two maternal isolation groups that were subjected to maternal isolation for 15 min or 3 hrs daily on postnatal days 2-17.On postnatal day 18,an amygdala kindling test was performed to induce seizures.The expression of GABAA receptor α1 in the hippocampus was determined by immunohistochemisty.Results The weights were reduced,the threshold of amygdala kindling and the stimulation number for full kindling decreased significantly,and seizures were more severe in the maternal isolation 3 hrs group compared with the normal control group.The expression of GABAA receptor α1 in the hippocampus CA1 area in the maternal isolation 3 hrs group decreased significantly compared with that in the normal groups.There were no significant differences in the aspects above mentioned between the maternal isolation 15 min and normal control groups.Conclusions The stress of early daily maternal isolation for 3 hrs may affect adversely brain development and increase epilepsy susceptibility in young rats.The decreased expression of GABAA receptor α1 in the hippocampus may contribute to the potential mechanism.
出处 《中国当代儿科杂志》 CAS CSCD 北大核心 2010年第8期662-665,共4页 Chinese Journal of Contemporary Pediatrics
关键词 应激 癫癎 易感性 GABAAα1 海马 幼鼠 Stress Epilepsy Susceptibility GABAA receptor α1 Hippocampus Young rats
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