摘要
目的 研究急性白血病患者组蛋白H3K4和H3K9甲基化及H3、H4乙酰化的修饰异常.方法 用Western blot方法检测19例急性白血病患者组蛋白H3K4、H3K9甲基化水平及15例急性白血病患者H3、H4乙酰化水平,同时选取9例非肿瘤患者及4名健康志愿者作为对照.结果 19例急性白血病患者组蛋白H3K4甲基化水平（0.220±0.096）低于对照组（0.447±0.186）（P＜0.01）;H3K9甲基化水平（0.409±0.106）高于对照组（0.168±0.015）（P＜0.01）.15例急性白血病患者组蛋白H3乙酰化水平（0.128±0.013）低于对照组（0.386±0.104）（P＜0.01）;H4乙酰化水平（0.096±0.008）低于对照组（0.341±0.096）（P＜0.01）.结论 急性白血病细胞的组蛋白甲基化、乙酰化修饰存在异常,表现为组蛋白H3K4甲基化水平减低,H3K9甲基化水平增高,组蛋白乙酰化水平呈明显的减低,这些表达的异常均与染色质结构致密、基因转录抑制有关.组蛋白甲基化、乙酰化调控修饰异常与急性白血病的发病可能有关,有可能作为急性白血病治疗的靶点.
Objective To study the aberration in histone H3K4 and H3K9 methylation and H3 and H4 acetylation in human acute leukemia（AL） cells.Methods The histone H3K4 and H3K9 methylation and H3 and H4 acetylation were detected by Western blot in 34 AL patients and 13 controls （9 non leukemia patients, 4 healthy volunteers）.Results The level of H3K4 methylation was significantly lower in 19 AL patients than in non leukemia（0.220±0.096 vs 0.447±0.186, P 〈 0.01 ）, while the level of H3K9 methylation was significantly higher （0.409±0.106 vs 0.168±0.015, P 〈 0.01 ） ; Both level of histone H3 and H4 acetylation in 15 AL patients were significantly lower （H3： 0.128±0.013 vs 0.386±0.104, H4：0.096±0.008 vs 0.341±0.096, respectively, both P 〈 0.01 ）.Conclusion Aberration of histone methylation and deficient histone acetylation in AL may represent the markers for an aberrant pest-translational modification of histones and chromatin structure.It might be a potential epigenetic target for anti-leukemia agent.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2010年第8期523-526,共4页
Chinese Journal of Hematology
基金
卫生部科学研究基金、福建卫生教育联合攻关计划（wkj2008-2-55）
福建医科大学科学研究发展专项基金计划（FZS08018）
漳州市科学研究发展计划基金（Z07014）
